In order to discuss the bryodiversity and bryoflora of Duoyihe Scenic Spot in Luoping County, Yunnan Province, the taxa and phytogeographical elements of the bryophyte were investigated and analyzed. The results showed that there were 116 species (including subspecies and varieties), belonging to 59 genera and 34 families in Duoyihe Scenic Spot. Both the dominant families and genera indicated that the bryoflora was mostly temperate elements with some subtropic and tropic elements. The areal-types were dominated by North Temperate (27.03%), Tropical Asia (25.23%), East Asia (19.82%), and endemic to China (10.81%); the dominant floristic elements are tropical elements (34.23%), northern temperate elements (35.14%) and eastern Asia elements (30.63%). Therefore, the characteristics of the bryoflora were that temperate and tropical elements coexist with high endemism. Compared with bryoflora in neighbouring 13 regions, the species abundance of Duoyihe was relatively high (ranked the eighth) and its bryoflora is the closest to Xiangzhigou karst areas in Yunnan-Guizhou Bryological Region due to the close geographic locations, the similar elevations and the same karst-river-valley environment in the two areas.