苦槛蓝叶的化学成分及其抑菌活性研究

Chemical Constituents from Leaves of Myoporum bontioides and Their Bacteriostatic Activities

摘要:

为研究苦槛蓝（Myoporum bontioides A. Gray）的化学成分，采用多种柱色谱技术从苦槛蓝叶中分离得到13 个化合物，它们的结构分别鉴定为：野黑樱苷（1）、 类叶升麻苷（2）、5，7-二羟基二氢黄酮（3）、3-O-β 谷甾醇苷（4）、（3R）-oct-1-en-3-ol-O-β-D-glucopyranosyl-（1"→2'）-O-β-D-glucopyranoside（5）、7-甲氧基香橙素（6）、 异樱花素（7）、 匙叶桉油烯醇（8）、 愈创木醇（9）、（1S，2R，5S，6R）-2，6-bis（5-methoxy-3，4-methylenedioxyphenyl）-3，7-dioxabicyclo[3.3.0]octane（10）、（1R，2S，5R，6S）-2-（4-hydroxy-3-methoxyphenyl）-6-（3，4-methylenedioxyphenyl）-3，7-dioxabicyclo[3.3.0]octane（11）、 去甲基川陈皮素（12）和3'，4'，5，6，7，8-六甲氧基黄酮（13），其中化合物4、5、7 ~ 11 为首次从苦槛蓝植物中分离得到。刃天青显色法测试部分化合物对大肠杆菌与金黄色葡萄球菌的抑制活性，结果表明，5，7-二羟基二氢黄酮（3）对大肠杆菌与金黄色葡萄球菌有一定的抑制作用，其MIC 值为62.50 μg mL^-1。

Abstract:

To study the chemical constituents in Myoporum bontioides A. Gray, thirteen compounds were isolated from the leaves of M. bontioides by various chromatography techniques. On the basis of spectral data, they were identified as prunasin (1), acteoside (2), 5,7-dihydroxyflavanone (3), anthemisol (4), (3R)- oct-1-en-3-ol-O-β-D-glucop-yranosyl-(1"→2")-O-β-D-glucopyranoside (5), 7-methoxyaromadenrin (6), 5,7-dihydroxy-2-(4-methoxyphenyl)-chroman-4-one (7), spainulenol (8), guaiol (9), (1S,2R,5S,6R)-2,6-bis(5- methoxy-3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane (10), (1R,2S,5R,6S)-2-(4-hydroxy-3- methoxyphenyl)-6-(3,4-methylenedioxyphenyl)-3,7-dioxabicyclo[3.3.0]octane (11), demethylnobiletin (12) and 2-(3,4-dimethoxyphenyl)-5,6,7,8-tetra-methoxy-4H-chromen-4-one (13). Compounds 4, 5, and 7 - 11 were obtained from M. bontioides for the first time. The inhibitory activity against Staphyloccocus aureus and Escherichia coil were determined by the use of a 96 well plate assay. The results showed that 5,7-dihydroxyflavone (3) possessed modest level of antibacterial activity with MIC value of 62.50 μg mL^-1.