枇杷果皮响应高温强光胁迫的蛋白质组分析

Proteomic Analysis of Loquat Peels under High Temperature and Strong Light Stresses

摘要:

为探讨枇杷[Eriobotrya japonica （Thunb.） Lindl.]果皮在高温强光胁迫下的蛋白质组分变化，采用蛋白质组学方法分析了果实日灼抗性差的枇杷种质‘WDYDB’果皮蛋白质对高温强光胁迫的应答反应。结果表明，在自然高温强光胁迫与遮光处理（对照）下，枇杷果皮蛋白质双向电泳图谱中表达量差异在2 倍以上的蛋白点共有31 个；通过MALDI-TOF-TOF/MS 质谱分析成功鉴定出26 个差异蛋白点，包括11 个下调蛋白和15 个上调蛋白。根据这些蛋白功能，可将其分为防御应答、碳水化合物和能量代谢、光合作用、其它等4 类蛋白。同时，对这些蛋白质在高温强光胁迫下的功能和作用进行了讨论。这些差异蛋白质参与了枇杷对高温强光胁迫的响应。

关键词:枇杷果皮;高温强光胁迫;日灼;差异表达蛋白

Abstract:

In order to understand the changes in protein components of loquat [Eriobotrya japonica (Thunb.) Lindl.] under high temperature and strong light stress, the responses of proteins in loquat ‘WDYDB' peels to stress were analyzed by using proteomic approaches, which fruits were sensitive to sunburn. The results showed that there were 31 protein dots with differential expression of more than two times on dimensional electrophoresis profiles, as compared between treatments of high temperature and strong light stresses and shading (Control). Among them, twenty-six differential proteins were identified by MALDI-TOF-TOF/MS analysis, including 11 down-regulated and 15 up-regulated proteins. These proteins could be divided into 4 categories according to their functions, including defense response, carbohydrate and energy metabolism, photosynthesis and other protein. The functions and roles of these proteins under high temperature and strong light stress were discussed. Therefore, it was suggested that these differential proteins could involve in responses to high temperature and strong light stresses.

Key words:Loquat peel;High temperature and strong light stresses;Sunburn;Differential expression protein