赤桉ACT1和ACT2基因的克隆与生物信息学分析

Cloning and Bioinformatics Analysis of ACT1 and ACT2 Genes from Eucalyptus camaldulensis

摘要:

为了解赤桉（Eucalyptus camaldulensis）肌动蛋白（Actin）在生长发育过程中的功能，根据赤桉幼苗转录组数据库中的肌动蛋白基因序列，从赤桉嫩叶中克隆了2 条Actin 基因片段，并利用RACE 技术获得Actin 基因的全长cDNA，分别命名为EC-ACT1和EC-ACT2 基因。生物信息学分析表明，这两条基因的全长cDNA 分别为1533 bp 和1387 bp，均含有1 个编码377 个氨基酸的开放阅读框。经比对分析，赤桉Actin 蛋白的氨基酸序列与其他植物Actin 蛋白的具有较高的相似性，并且具有Actin蛋白特有的保守序列和相关特征。因此推测这两条基因对桉树的生长发育具有一定的调控作用。

Abstract:

In order to reveal the biological function of Actin in growth and development of Eucalyptus camaldulensis, two fragments were cloned from the transcriptome database of E. camaldulensis seedlings at different developmental stages, named as EC-ACT1 and EC-ACT2, and their full-length cDNA were obtained by RACE method. Bioinformatics analysis showed that the full-length cDNA of EC-ACT1 and EC-ACT2 were 1533 bp and 1387 bp, respectively, and both with 1131 bp ORF encoding 377 amino acids. The amino acid sequence of Actin in E. camaldulensis had high similarity to those of other plants with typical conserved domains of Actin. Therefore, the EC-ACT1 and EC-ACT2 genes might play a role in regulating growth and development of E. camaldulensis.