牛鞭草品种EST-SSR指纹图谱构建及遗传多样性分析
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四川农业大学草业科学系,四川农业大学草业科学系,四川农业大学草业科学系,四川农业大学草业科学系,四川农业大学草业科学系,四川农业大学草业科学系,四川农业大学草业科学系,四川农业大学草业科学系

基金项目:

四川省育种攻关项目(2011YZGG-11);国家现代牧草产业技术体项目(CARS-35-05);教育部博士点新教师基金项目(20115103120004); 四川农业大学大学生科研兴趣计划项目(20131155)资助


Construction of Fingerprinting and Genetic Diversity Analysis of Hemarthia Cultivars by EST-SSR
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Department of Grassland Science, Sichuan Agricultural University,Department of Grassland Science, Sichuan Agricultural University,Department of Grassland Science, Sichuan Agricultural University,Department of Grassland Science, Sichuan Agricultural University,Department of Grassland Science, Sichuan Agricultural University,Department of Grassland Science, Sichuan Agricultural University,Department of Grassland Science, Sichuan Agricultural University,Department of Grassland Science, Sichuan Agricultural University

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    摘要:

    为探讨牛鞭草(Hemarthria spp.)品种间的遗传多样性,从86 对EST-SSR 引物中筛选出8 对引物对牛鞭草属6 个品种进行指纹图谱的构建及遗传多样性分析。结果表明,8 对EST-SSR 引物对牛鞭草属6 个品种共扩增出193 条清晰条带,多态性条带161 条,多态性比例为83.4%。每条引物的多态信息含量(PIC)为0.480 ~ 0.695,平均为0.602。UPGMA 聚类分析表明,牛鞭草属6 个品种在相似系数为0.652 处可分为两大类群。8 对EST-SSR 引物均能将6 个品种完全区分开,以3 对EST-SSR 引物扩增的电泳图谱为基础,建立了牛鞭草属6 个品种的指纹图谱标准模式图,每个品种都有唯一的指纹图谱。牛鞭草属6 个品种的平均Nei’s 基因多样性指数为0.333,平均Shannon 信息指数为0.496,品种间的相似系数介于0.399 ~ 0.782 之间。可见,牛鞭草属植物品种的遗传多样性较丰富,种间差异明显。

    Abstract:

    In order to understand the genetic diversity of Hemarthria cultivars, the DNA fingerprints of six Hemarthia cultivars were constructed by using 8 pairs of EST-SSR primers screened from 86 pairs, and the genetic diversities among them were analyzed. The results showed that eight pairs of ESR-SSR primers totally amplified 194 bands, in which 161 bands were polymorphic bands, accounting for 83.4%. The polymorphic information content (PIC) ranged from 0.480 to 0.695, with an average of 0.602. The UPGMA analysis showed that six cultivars of Hemarthia could be divided into two groups at the similarity coefficient of 0.652, and 6 cultivars could be completely separated by eight pairs of ESR-SSR primers. Based on electrophoretogram amplified by 3 pairs primers, the standard DNA fingerprinting for 6 Hemarthia cultivars were constructed, and each cultivars had an unique fingerprint. The average Nei's genetic diversity and Shannon's information index were 0.333 and 0.496, respectively. The similarity coefficient of 6 cultivars of Hemarthia ranged from 0.399 to 0.782. Therefore, it was suggested that 6 cultivars of Hemarthia had rich genetic diversity and significant differences between species.

    参考文献
    [1] Delectis Florae Reipublicae Popularis Sinicae Agendae Academiae Sinicae Edita. Florae Reipublicae Popularis Sinicae, Tomus 10(2)[M]. Beijing: Science Press, 1997: 261-263. 中国植物志编辑委员会. 中国植物志, 第10卷第2分册 [M]. 北 京: 科学出版社, 1997: 261-263.
    [2] Yang C H, Zhang X Q, Li X L, et al. Study on the agricultural traits and breeding of limpograss (Hemarthria R. Br.) [J]. J Sichuan Grass, 2004(9): 8-11. 杨春华, 张新全, 李向林, 等. 牛鞭草资源农艺性状和育种研究[J]. 四川草原, 2004(9): 8-11.
    [3] Yang C H, Zhang X Q, Li X L, et al. Hemarthria germplasm resources and breeding [J]. Acta Prat Sin, 2004, 13(2): 7-12. 杨春华, 张新全, 李向林, 等. 牛鞭草属种质资源及育种研究[J]. 草业学报, 2004, 13(2): 7-12.
    [4] Quesenberry K H, Dunavin L S, Hodges E M, et al. Registration of redalta, greenalta, and bigalta limpograss1 (reg. Nos. 52, 53, and 54) [J]. Crop Sci, 1979, 19(2): 294-294.
    [5] Quesenberry K H, Ocumpaugh W R, Ruelke O C, et al. Registration of ‘Floralta' limpograss [J]. Crop Sci, 1987, 27(5): 1087-1087.
    [6] de Carvalho D D, Goncalez D D, Ghisi O M A A. Agronomic evaluation of seven accessions of Hemarthrla altissima (Poir.) Stapf et CE Hubb in Pindamonhangaba, Sao Paulo [J]. Rev Soc Brasil Zootecn, 1996, 25(5): 825-836.
    [7] Woods P W, Couchman J N, Barlow H A. Variation and adaptation in limpograss (Hemarthria altissima) [J]. Proc New Zealand Grassland Assoc, 1996, 57(1): 49-53.
    [8] Rumball P J, Lambert J P. Plant introduction trials: Performance of some subtropical grasses in Northland [J]. New Zealand J Exp Agri, 1981, 9(1): 79-83.
    [9] Cai Y B, Yang X Y, Chen X G, et al. Establishment and application of SCoT amplification system for macadamia [J]. J Trop Subtrop Bot, 2013, 21(3): 253-258. 蔡元保, 杨祥燕, 陈显国, 等. 澳洲坚果SCoT反应体系的建立及 应用 [J]. 热带亚热带植物学报, 2013, 21(3): 253-258.
    [10] Bai J, Hu X, Zhou S T, et al. Genetic diversity of 47 Cymbidium ensifolium varieties assessed by SRAP markers [J]. J Plant Genet Resour, 2012, 13(3): 376-380. 白坚, 胡旭, 周淑婷, 等. 47个建兰品种的SRAP遗传多样性分 析 [J]. 植物遗传资源学报, 2012, 13(3): 376-380.
    [11] Xue D, Xiao L, Ai X, et al. Genetic diversity of Miscanthus floridulus revealed by morphological characters and SSR markers [J]. Acta Prat Sin, 2012, 21(5): 96-106. 薛德, 肖亮, 艾辛, 等. 五节芒表型性状和SSR标记遗传多样性 分析 [J]. 草业学报, 2012, 21(5): 96-106.
    [12] Liu J, Zhao Q, Yang Z M. ISSR molecular markers analysis 9 bermudagrass cultivation varieties [J]. Acta Prat Sin, 2012, 21(6): 159-165. 刘君, 赵琴, 杨志民. ISSR分子标记对9种狗牙根的鉴定分析[J]. 草业学报, 2012, 21(6): 159-165.
    [13] Ge Y Y, Zhang F, Shen X L, et al. Analysis of genetic diversity and construction of fingerprint of Vriesea by ISSR [J]. Sci Agri Sin, 2012, 45(4): 726-733. 葛亚英, 张飞, 沈晓岚, 等. 丽穗凤梨ISSR遗传多样性分析与指 纹图谱构建 [J]. 中国农业科学, 2012, 45(4): 726-733.
    [14] Wu Y T, Zhang T Z, Guo W Z, et al. Detecting polymorphism among up land cotton (Gossypium hirsutum L.) cultivars and their roles in seed purity of hybrids with SSR markers [J]. Cott Sci, 2001, 13(3): 131-133. 武耀廷, 张天真, 郭旺珍, 等. 陆地棉品种SSR标记的多态性 及用于杂交种纯度检测的研究 [J]. 棉花学报, 2001, 13(3): 131-133.
    [15] Kojima T, Nagaoka T, Noda K, et al. Genetic linkage map of ISSR and RAPD markers in einkorn wheat in relation to that of RFLP markers [J]. Theor Appl Genet, 1998, 96(1): 37-45.
    [16] Shao Y J, Wu W W, Shen S X, et al. Genetic study on genic male sterility of pepper and its application in hybrid breeding [J]. J Trop Subtrop Bot, 2013, 21(1): 93-99. 邵元健, 吴雯雯, 沈素香, 等. 辣椒雄性核不育基因的遗传研 究及其在杂交育种中的应用 [J]. 热带亚热带植物学报, 2013, 21(1): 93-99.
    [17] Gupta P K, Balyan H S, Sharma P C, et al. Microsatellites in plants: A new class of molecular markers [J]. Curr Sci, 1996, 70(1): 45-54.
    [18] Holton T A, Christopher J T, McClure L, et al. Identification and mapping of polymorphic SSR markers from expressed gene sequences of barley and wheat [J]. Mol Breed, 2002, 9(2): 63-71.
    [19] Zhang L Y, Bernard M, Leroy P, et al. High transferability of bread wheat EST-derived SSRs to other cereals [J]. Theor Appl Genet, 2005, 111(4): 677-687.
    [20] Saha M C, Rouf M M A, Eujayl I, et al. Tall fescue EST-SSR markers with transferability across several grass species [J]. Theor Appl Genet, 2005, 109(4): 783-791.
    [21] Thiel T, Michalek W, Varshney R, et al. Exploiting EST databases for the development and characterization of genederived SSR-markers in barley (Hordeum vulgare L.) [J]. Theor Appl Genet, 2003, 106(3): 411-422.
    [22] Chen Y X, Zhang X Q, Xie W G, et al. Genetic diversity of Hemarthria compressa germplasms from southwestern china based on EST-SSR markers [J]. Acta Prat Sin, 2011, 2(6): 245- 253. 陈永霞, 张新全, 谢文刚, 等. 利用EST-SSR标记分析西南扁穗 牛鞭草种质的遗传多样性 [J]. 草业学报, 2011, 2(6): 245-253.
    [23] Fan Y, Li F, Zhang X Q, et al. Genetic diversity of Hemarthria compressa germplasm detected by inter-simple sequence repeat (ISSR) [J]. Acta Prat Sin, 2007, 16(4): 76-81. 范彦, 李芳, 张新全, 等. 扁穗牛鞭草种质遗传多样性的ISSR 分析 [J]. 草业学报, 2007, 16(4): 76-81.
    [24] Fan Y, Xu Y D, Jiang A, et al. Genetic diversity of Hemarthria compressa germplasms in southwest China by SRAP analysis [J]. Genom Appl Biol, 2010, 29(1): 63-70. 范彦, 徐远东, 蒋安, 等. 中国西南区扁穗牛鞭草种质遗传多 样性的SRAP分析 [J]. 基因组学与应用生物学, 2010, 29(1): 63-70.
    [25] Kantety R V, Rota M L, Matthews D E, et al. Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat [J]. Plant Mol Biol, 2002, 48(5/6): 501-510.
    [26] Wei Z W. DNA fingerprint of Medicago sativa variety genomes using SSR, ISSR and RAPD [J]. Acta Prat Sin, 2004, 13(3): 62-67. 魏臻武. 利用SSR、ISSR和RAPD技术构建苜蓿基因组DNA 指纹图谱 [J]. 草业学报, 2004, 13(3): 62-67.
    [27] Shi J, Song B T, Jin K J, et al. Genetic diversity and fingerprinting of 50 pigmented potato (Solanum tuberosum L.) genetypes with SSR markers [J]. J Agri Biotechn, 2012, 20(4): 362-371. 石景, 宋波涛, 金开建, 等. SSR标记的彩色马铃薯遗传多样 性分析及指纹图谱构建 [J]. 农业生物技术学报, 2012, 20(4): 362-371.
    [28] Liu L W, Zhao L P, Gong Y Q, et al. DNA fingerprinting and genetic diversity analysis of late-bolting radish cultivars with RAPD, ISSR and SRAP markers [J]. Sci Hort, 2008, 116(3): 240-247.
    [29] Teng H T, Lü B, Zhao J R, et al. DNA fingerprint profile involved in plant variety protection practice [J]. Biotechn Bull, 2009(1): 1-6. 腾海涛, 吕波, 赵久然, 等. 利用DNA指纹图谱辅助植物新品 种保护的可能性 [J]. 生物技术通报, 2009(1): 1-6.
    [30] Li L, He W M, Ma L P, et al. Construction chinese cabbage (Brassica rapa L.) core collection and its EST-SSR fingerprint database by EST-SSR molecular markers [J]. Genom Appl Biol, 2009, 28(1): 76-88. 李丽, 何伟明, 马连平, 等. 用EST-SSR分子标记技术构建大 白菜核心种质及其指纹图谱库 [J]. 基因组学与应用生物学, 2009, 28(1): 76-88.
    [31] Xu Z D, Zhao L Y, Zhang L, et al. Analysis of genetic diversity and construction of fingerprint of Rosa rugosa by SRAP [J]. Sci Agri Sin, 2011, 44(8): 1662-1669. 徐宗大, 赵兰勇, 张玲, 等. 玫瑰SRAP遗传多样性分析与品种 指纹图谱构建 [J]. 中国农业科学, 2011, 44(8): 1662-1669.
    [32] Tong H L, Feng S P, He J H, et al. Establishment of fingerprinting for pineapple (Ananas comosus) by SSR marker [J]. J Fruit Sci, 2011, 28(2): 240-245. 童和林, 冯素萍, 贺军虎, 等. 菠萝微卫星指纹图谱的构建 [J]. 果树学报, 2011, 28(2): 240-245.
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黄秀,曾捷,聂刚,刘欢,黄琳凯,张新全,蒋晓梅,张瑜,张博涛.牛鞭草品种EST-SSR指纹图谱构建及遗传多样性分析[J].热带亚热带植物学报,2014,22(2):165~171

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  • 收稿日期:2013-07-19
  • 最后修改日期:2013-10-18
  • 录用日期:2013-11-25
  • 在线发布日期: 2014-03-26
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