黄皮果皮中的抗菌活性成分研究

Antibacterial Components from Peels of Clausena lansium (Lour.) Skeels

摘要:

为了解黄皮[Clausena lansium（Lour.）Skeels]果皮中的抗菌活性成分，采用硅胶柱色谱、Sephadex LH-20 凝胶柱色谱等方法，从黄皮果皮的乙醇提取物中分离得到10 个化合物，经波谱学分析鉴定为： lansine（1）、3-甲酰基咔唑（2）、3-甲酰基-6-甲氧基咔唑（3）、6-甲氧基咔唑-3-羧酸甲酯（4）、（6R，7E，9S）-9-羟基-4，7-巨豆二烯-3-酮（5）、7-羟基香豆素（6）、8-羟基呋喃香豆素（7）、辛黄皮酰胺（8）、对羟基肉桂酸甲酯（9）和胡萝卜苷（10）。其中化合物5 和9 为首次从黄皮属植物中分离得到，化合物8 的碳谱数据是首次报道。用滤纸片琼脂扩散法测定化合物的抗菌活性，结果表明，化合物 1、2、3、6、7 和9 对金黄色葡萄球菌（Staphylococcus aureus）有抑制作用。这有利于更好地开发利用黄皮这一药食两用的水果资源。

Abstract:

In order to understand the chemical constituents of Clausena lansium (Lour.) Skeels, ten compounds were isolated from 95% EtOH extract of peels ofC. lansiumby silica gel and sephadex LH-20 column chromatography. On the basis of spectral data, their structures were identified as lansine (1), 3-formyl carbazole (2), 3-formyl- 6-methoxy carbazole (3), 6-methoxycarbazole-3-carboxylate (4), (6R,7E,9S)-9-hydroxy-4,7-megastigmadien- 3-one (5), umbelliferone (6), 8-hydroxyfurocoumarin (7), zeta-clausenamide (8), methyl p-hydroxycinnamate (9), and daucosterol (10). Compounds 5 and 9 were isolated from the genus Clausena for the first time, and the ¹³C NMR spectral data of compound 8 was also reported for the first time. Compounds 1, 2, 3, 6, 7 and 9 showed antibacterial activity on Staphylococcus aureus in a bioactive assay by using paper disk diffusion method. These would be helpful for exploitation of Clausena lansium.