不同培养条件对勺叶茅膏菜试管苗矶松素含量的影响

Effect of in vitro Culture Conditions on Content of Plumbagin in Sundew (Drosera spatulata) Plantlets

摘要:

为探讨培养条件对勺叶茅膏菜（Drosera spatulata）试管苗矶松素积累的影响，采用高效液相色谱（HPLC）法测定矶松素含量，对不同器官和不同培养条件下的勺叶茅膏菜试管苗矶松素含量变化进行研究。结果表明，勺叶茅膏菜试管苗根的矶松素含量显著高于叶片；光质和有机物含量对勺叶茅膏菜试管苗矶松素含量的影响不显著，但对试管苗的生长具有显著影响，最佳培养光质为白光，其次为红光和蓝光，最后为绿光；适当降低培养基中有机物含量可促进勺叶茅膏菜试管苗的生长发育；植物生长调节剂对矶松素积累的影响效应依次为6-BA > NAA > KT > GA₃，而对试管苗生长的影响效应依次为6-BA > GA₃ > NAA >KT。因此，勺叶茅膏菜试管苗的最佳培养条件为：以1/2MS 为基本培养基，添加 0 ~ 0.2 mg L^-1 6-BA、0.2 mg L^-1 NAA、0.5 mg L^-1KT 和0.1 mg L^-1 GA₃，于白光下培养。

Abstract:

In order to understand the effect of different culture conditions on content of plumbagin in sundew (Drosera spatulata) plantlets, the changes in plumbagin content were studied by using high performance liquid chromatography (HPLC). The results showed that the content of plumbagin in roots was higher than that in leaves. Light quality and organic element content in medium had no significant differences in plumbagin accumulation in sundew plantlets, but had significant effects on its growth. White light was the best for culture of sundew plantlets, followed by red light, blue light, and green light. Appropriate to reduce the organic matter content in medium could promote the growth and development of sundew plantlets. The effects of different plant growth regulator on plumbagin accumulation were in the order of 6-BA > NAA > KT > GA₃, and those on growth were in descending order: 6-BA > GA₃ > NAA > KT. Therefore, the optimum culture condition for sundew plantlets was 1/2MS as basic medium under white light, supplemented with 0 - 0.2 mg L^-1 6-BA, 0.2 mg L^-1 NAA, 0.5 mg L^-1 KT and 0.1 mg L^-1 GA₃.