成年态南丰蜜橘试管嫁接育苗技术研究

Studies on in vitro Micrografting of Mature Citrus reticulata Blanco ‘Kinokuni’ (Tanaka) H. H. Hu

摘要:

为探索适合成年态南丰蜜橘[Citrus reticulata Blanco ‘kinokuni’（Tanaka）H. H. Hu]的快速繁殖技术，对其试管茎尖微嫁接育苗进行研究。结果表明，最好的砧木是苦柚种子苗，以腹接方式的成活率最高。嫁接苗接种在MS + GA₃ 1 mg L^-1 + 蔗糖75 g L^-1 的培养基中，暗培养7 d 后转入光周期下培养，嫁接成活率达67.78%。不同移栽基质对嫁接苗的成活率影响不显著。嫁接苗与成年态南丰蜜橘再生芽在形态和POD、CAT 及SOD 同工酶分子表达上均无明显差异。这表明通过试管茎尖微嫁接技术可保持其遗传稳定性。

Abstract:

In order to exploe the rapid propagation of mature Citrus reticulata Blanco ‘Kinokuni’ (Tanaka) H. H. Hu, the breeding technology by in vitro micrografting was studied. The results showed that the most suitable rootstock was bitter pomelo [C. maxima (Burm.) Merr. ‘Maxima’] seedling and the side grafting method had the highest survival. The micrografting seedlings cultured in MS medium with 1 mg L^-1 GA₃ and 75 g L^-1 sugar were at first grown in dark for 7 days, then transferred to light with 10 h d^-1, which survival cultured after 25 days reached 67.78%. The transplanting substrate had not significant influence on survival of grafting seedlings. Furthermore, there were no obvious differences between grafting seedlings and adventitious buds in morphology, as well as the isozyme expression of POD, CAT and SOD. It was suggested that the in vitro micografting technique could keep the genetic stability of grafted seedlings.