氚标记胸腺嘧啶掺入法在土壤细菌生长速率研究中的应用

Applications of Tritiated Thymidine Incorporation in Determination of Bacterial Growth Rate

摘要:

氚标记胸腺嘧啶掺入法因其原理明确、方法简单易行、重复性好等特点在测定细菌生长速率中发挥着重要的作用。然而，相比较医学和水生生态学领域，该方法在土壤生态学研究中的应用还较少，尤其是我国此方面的研究尚未见报道。就氚标记胸腺嘧啶掺入法的原理、操作流程、影响因素、计算方法及其在土壤生态学中的应用等方面进行了综述，为该方法在我国土壤生态学领域的推广应用提供参考。

Abstract:

Tritiated thymidine incorporation method plays an important role in determination of bacterial growth rate, due to its clear principle, simple operation and good repeatability. However, there has been little research on soil ecology using tritiated thymidine incorporation method for determination of bacterial growth rate compared with medical science and aquatic ecology field. Especially studies on this area in China have been reported hardly. The principle, operation procedure, influential factor and calculation method of tritiated thymidine incorporation method were introduced, and its applications in soil ecology were summarized. These would provide reference for the further applications of this technology in soil ecology.