黄花梨bZIP的cDNA克隆与表达分析

Cloning and Expresssion Analysis of bZIP Gene from Pyrus pyrifolia ‘Huanghua’

摘要:

为探讨黄花梨（Pyrus pyrifolia ‘Huanghua’）PpbZIP 基因的功能，根据同源基因bZIP 从黄花梨花芽中克隆到PpbZIP 基因（GenBank 登录号： KC951877）。PpbZIP 基因的全长cDNA 为1843 bp，其中开放阅读框长度1227 bp，编码408 个氨基酸，与同为蔷薇科的苹果bZIP 蛋白序列的同源性高达92%。PpbZIP 基因的QRT-PCR 分析表明，PpbZIP 基因在黄花梨花芽从休眠到休眠解除后的表达量呈先升高后降低的趋势。这表明PpbZIP 参与了黄花梨花芽休眠过程的调控。

Abstract:

In order to understand the function of PpbZIP of Pyrus pyrifolia ‘Huanghua’, PpbZIP gene was cloned from flower buds of ‘Huanghua’ based on homologous gene of bZIP. The results showed that full length of cDNA of PpbZIP (GenBank accession No.: KC951877) was 1843 bp, containing an ORF of 1227 bp and encoding 408 amino acids. PpbZIP also shared 92% homology with bZIP of Malus × domestica. QRT-PCR analysis showed that the expression of PpbZIP gene increased at initiate dormancy stage and then decreased with dormancy releasing. It was suggested that PpbZIP could involve in the regulation of bud dormancy process of ‘Huanghua’.