水稻组蛋白脱乙酰化酶基因HDA705启动子的克隆与表达特性分析

Cloning and Expression Characterization of Histone Deacetylase Gene HDA705 Promoter in Rice (Oryza sativa)

摘要:

为探讨水稻（Oryza sativa）组蛋白脱乙酰化酶基因HDA705 的功能和表达特性，根据NCBI 上登录的水稻HDA705 基因（GenBank 登录号：AK111861）的序列，克隆了5′ 端2 kb 的启动子片段proHDA705，并构建了proHDA705：GUS 表达载体。通过农杆菌介导法转化水稻，并获得了转基因株系。GUS检测结果表明，proHDA705 仅在水稻的根、茎、叶及部分颖壳的表皮毛等器官中表达，而在花器官中不表达，这表明HDA705 具有组织表达特异性。

Abstract:

In order to understand the function and expression character of histone deacetylase gene HDA705 in rice (Oryza sativa), 5′ terminal promoter sequence was cloned with length of 2 kb based on rice HDA705 sequence (GenBank accession No.: AK111861), named proHDA705. The expression vector of proHDA705:GUS was constructed. The proHDA705:GUS vector was transformed to rice by Agrobacterium-mediated assays. GUS staining assays of transgenic rice showed that proHDA705 expressed only in root, stem, leave and some epidermal hair of seed coat, but did not in flower. It was suggested that HDA705 had tissue specific expression in rice.