栀子八氢番茄红素合成酶基因的分离及表达分析

Isolation and Expression of Phytoene Synthase Gene in Gardenia jasminoides

摘要:

为探讨栀子(Gardenia jasminoides)果实中藏花素的合成机理,克隆了栀子类胡萝卜素生物合成的关键酶八氢番茄红素合成酶(GjPSY)基因的全长cDNA。结果表明,推导的GjPSY 氨基酸序列与双子叶植物来源的GjPSY 亲缘关系较近。采用HPLC 检测栀子果实中的藏花素-1 含量为(3.96±1.48) mg g^-1,在叶片中未检出。通过RT-PCR 分析表明,GjPSY 在栀子叶片和果实中均有表达,且表达水平一致。因此推测,GjPSY 的转录水平与果实中藏花素-1 的合成无关。

Abstract:

In order to understand the synthesis mechanism of crocin in Gardenia jasminoides fruits, the full-length cDNAs of phytoene synthase gene GjPSY from G. jasminoides were cloned. The results showed that the deduced amino acid sequence of GjPSY had close relation with those from dicotyledonsous plants. The content of crocin-1 in fruits was (3.96±1.48) mg g^-1 by HPLC, while crocin-1 was not detected in leaves. It was revealed that crocin-1 exclusively accumulated in fruits. The RT-PCR analysis revealed that the expression of GjPSY in fruits was not differences from that in leaves. Therefore, it was suggested that the crocin synthesis in G. jasminoides fruits had not correlation with transcription level of GjPSY gene.