紫茉莉悬浮细胞培养体系的建立

Studies on Establishment of Suspension Cell System for Mirabilis jalapa L.

摘要:

为建立紫茉莉(Mirabilis jalapa L.)悬浮细胞培养体系,以紫茉莉无菌苗叶片诱导的愈伤组织为材料,筛选紫茉莉悬浮细胞的适宜培养体系。结果表明,紫茉莉愈伤组织在MS + 2,4-D 1 mg L^-1 + KT 0.5 mg L^-1 的液体培养基中悬浮继代培养3~4 次,能得到稳定的悬浮细胞系。培养基的pH 值为5.5~5.9,蔗糖浓度为30 g L^-1 更适合悬浮细胞的生长。紫茉莉悬浮细胞的生长曲线大致呈S 型。最佳继代培养时间是10 d,培养液的体积为40 mL 时,接种量为7.5 mL,可以较好地保持悬浮细胞系。1 L培养液中可提取分泌蛋白(0.42±0.15) g。这些有助于对悬浮细胞提取分泌蛋白的研究。

Abstract:

In order to establish suspension cell system of Mirabilis jalapa L., the calli induced from leaves of aseptic seedlings of M. jalapa were used as material, the optimum suspension culture system was studied. The results showed that the stable suspension cell system could be obtained after calli were subcultured 3-4 times on MS medium with 2,4-D 1 mg L^-1 and KT 0.5 mg L^-1. The optimum pH was 5.5-5.9 with 30 g L^-1 sucrose. The growth curve of suspension cells of M. jalapa was S-type, and subculture time was about 10 days. The optimum inoculum was 7.5 mL in 40 mL medium. Secretory protein was (0.42±0.15) g extracted from 1 L medium. These were useful for studying secretory protein extracted from suspension system.