基于5.8S rDNA-ITS序列的我国浙江沿海铜藻群体遗传多样性分析
作者:
作者单位:

温州医学院生命科学学院,浙江省海洋水产养殖研究所,浙江省海洋水产养殖研究所,温州医学院生命科学学院,温州医学院生命科学学院

基金项目:

国家海洋公益性行业科研专项经费项目(201105008);浙江省海洋与渔业项目;浙江省省属科研院所扶持专项计划项目(2012F20028)资助


Genetic Analysis Based on 5.8S rDNA-ITS Sequence of Sargassum horneri (Turner) C. Agardh in the Coastal Areas of Zhejiang Province, China
Author:
Affiliation:

School of Life Science,Wenzhou Medical College,Zhejiang Mariculture Research Institute,Zhejiang Mariculture Research Institute,School of Life Science,Wenzhou Medical College,School of Life science, Wenzhou Medical College

  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [31]
  • |
  • 相似文献 [20]
  • | | |
  • 文章评论
    摘要:

    为了调查我国浙江沿海铜藻(Sargassum horneri)群体遗传多样性,对浙江省南麂岛火焜岙、洞头岛、枸杞岛的野生群体和南麂岛马祖岙养殖群体的铜藻44个样品的5.8S rDNA-ITS序列进行了PCR扩增和序列分析,获得DNA片段长度为1485 bp,包括部分ITS1、完整的5.8S rDNA和部分ITS2序列。序列分析表明仅有1个变异位点,说明群体间没有明显的遗传分化,遗传多样性较低。另外,将包含这一变异位点的基因型与GenBank公共数据库中另外2株铜藻的5.8S rDNA-ITS区序列进行比对,共有31个变异位点,其中插入/缺失位点10个。因此,建议加快人工铜藻场的建设以及野生铜藻场的恢复,保持铜藻场的生态平衡。

    Abstract:

    The purpose was to investigate genetic diversity of Sargassum horneri populations in the coastal areas of Zhejiang Province, China. The 5.8S rDNA-ITS sequences from 44 individuals of S. horneri were amplified by PCR and analyzed, including wild populations from Huokun'ao of Nanji Island, Dongtou Island and Gouqi Island and Mazuao cultured population from Nanji Island in Zhejiang Province. The whole-length of sequence from all individuals was 1485 bp, including partial ITS1, ITS2 sequences, and complete 5.8S rDNA sequence. Among them, there was only one variable nucleotide site. It was suggested that there was no distinct genetic differentiation among populations with relatively low genetic diversity. In addition, 5.8S rDNA-ITS sequences from two haplotypes with the only one variable nucleotide site and another two sequences from GenBank public database were aligned, 31 variable nucleotide sites were detected, and 10 of them were insert or delete sites. Therefore, it was proposed to speed up the construction of artificial S. horneri field and restore wild S. horneri field in order to maintain an ecological balance.

    参考文献
    [1] Zeng C K, Lu B R. Phaeophyta: Fucales [M]// Flora Algarum Marinarum Sinicarum, Tomus 3(2). Beijing: Science Press, 2000: 1-43. 曾呈奎, 陆保仁. 褐藻门: 墨角藻目 [M]// 中国海藻志 第3(2)卷. 北京: 科学出版社, 2000: 1-43.
    [2] Sun J Z, Zhuang D G, Chen W D, et al. Studies on sexual reproduction and seedling production of the brown alga Sargassum horneri [J]. S China Fish Sci, 2008, 4(2): 6-14. 孙建璋, 庄定根, 陈万东, 等. 铜藻Sargassum horneri繁殖生物 学及种苗培育研究 [J]. 南方水产, 2008, 4(2): 6-14.
    [3] Sun J Z, Zhuang D G, Wang T G, et al. Study on Sargassum horneri (Turner) C. Agardh around Nanji Islands [J]. Mod Fish Inform, 2009, 24(5): 19-21. 孙建璋, 庄定根, 王铁杆, 等. 南麂列岛铜藻的研究 [J]. 现代渔 业信息, 2009, 24(5): 19-21.
    [4] Sun J Z, Zhuang D G, Wang T G, et al. Primary study on enhancement technique of Sargassum horneri around Nanji Islands [J]. Mod Fish Inform, 2010, 25(1): 23-27. 孙建璋, 庄定根, 王铁杆, 等. 南麂列岛铜藻增殖技术的初步研 究 [J]. 现代渔业信息, 2010, 25(1): 23-27.
    [5] Umezaki I. Ecological studies of Sargassum horneri (Turner) C. Agardh in Obama Bay, Japan Sea [J]. Bull Jpn Soc Sci Fish, 1984, 50(7): 1193-1200.
    [6] Choi H G, Lee K H, Yoo H I, et al. Physiological differences in the growth of Sargassum horneri between the germling and adult stages [J]. J Appl Phycol, 2008, 20(5): 279-285.
    [7] Honda M, Okuda T. Egg liberation, germling development and seasonal changes in photosynthetic rates of autumnal Sargassum horneri [J]. Jpn J Phycol, 1989, 37(1): 46-52.
    [8] Uchida T. The life cycle of Sargassum horneri (Phaeophyta) in laboratory culture [J]. J Phycol, 1993, 29(2): 231-235.
    [9] Yoshida G, Murase N, Arai S, et al. Ecotypic differentiation in maturation seasonality among Sargassum horneri (Fucales, Phaeophyta) populations in Hiroshima Bay, Seto Inland Sea, Japan [J]. Phycologia, 2004, 43(6): 703-710.
    [10] Pang S J, Liu F, Shan T F, et al. Cultivation of the brown alga Sargassum horneri: Sexual reproduction and seedling production in tank culture under reduced solar irradiance in ambient temperature [J]. J Appl Phycol, 2009, 21(4): 413-422.
    [11] Ogawa H. Combined effects of temperature and salinity on the early development of marine algae: II. Rhizoid development of Sargassum horneri (Turner) C. Agardh [J]. Jpn J Phycol, 1986, 34(2): 137-141.
    [12] Uchida T, Arima S, Hirata S, et al. Free-living cells released from thalli of Sargassum horneri (Phaeophyta) in culture [J]. Bot Mar, 1996, 39(1/2/3/4/5/6): 269-272.
    [13] Oak J H, Suh Y, Lee I K. Phylogenetic relationships of Sargassum subgenus Bactrophycus (Sargassaceae, Phaeophyceae) inferred from rDNA ITS sequences [J]. Algae, 2002, 17(4): 235-247.
    [14] Uwai S, Kogame K, Yoshida G, et al. Geographical genetic structure and phylogeography of the Sargassum horneri / filicinum complex in Japan, based on the mitochondrial COXⅢ haplotype [J]. Mar Biol, 2009, 156(5): 901-911.
    [15] Hu Z M, Uwai S, Yu S H, et al. Phylogeographic heterogeneity of the brown macroalga Sargassum horneri (Fucaceae) in the northwestern Pacific in relation to late Pleistocene glaciation and tectonic configurations [J]. Mol Ecol, 2011, 20(18): 3894-3909.
    [16] Hillis D M, Dixon M T. Ribosomal DNA: Molecular evolution and phylogenetic inference [J]. Q Rev Biol, 1991, 66(4): 411-453.
    [17] Niu X L, Ji K P, Wu Q, et al. rDNA ITS sequence molecular marker technology in the study of botany [J]. China J Bioinform 2009, 7(4): 268-271. 牛宪立, 姬可平, 吴群, 等. rDNA ITS区序列分子标记技术在植 物学研究中的应用 [J]. 生物信息学, 2009, 7(4): 268-271.
    [18] Baldwin B G, Sanderson M J, Porter J M, et al. The ITS region of nuclear ribosomal DNA: A valuable source of evidence on angiosperm phylogeny [J]. Ann Missouri Bot Gard, 1995, 82(2): 247-277.
    [19] Ainouche M L, Bayer R J. On the origins of the tetraploid Bromus species (section Bromus, Poaceae): Insights from internal transcribed spacer sequences of nuclear ribosomal DNA[J]. Genome, 1997, 40(5): 730-743.
    [20] Godoy-Lutz G, Kuninaga S, Steadman J R, et al. Phylogenetic analysis of Rhizoctonia solani subgroups associated with web blight symptoms on common bean based on ITS-5.8S rDNA [J]. J Gen Plant Pathol, 2008, 74(1): 32-40.
    [21] Martins L, Oberprieler C, Hellwig F H. A phylogenetic analysis of Primulaceae s.l. based on internal transcribed spacer (ITS) DNA sequence data [J]. Plant Syst Evol, 2003, 237(1/2): 75-85.
    [22] Sun G H, Liu X Q, Yang J M, et al. Genetic diversity of ITS sequences in farmed and natural Rhopilema esculentum populations [J]. Mar Sci, 2010, 34(10): 90-95. 孙国华, 刘相全, 杨建敏, 等. 海蜇养殖群体及自然捕获群体 ITS序列遗传分析 [J]. 海洋科学, 2010, 34(10): 90-95.
    [23] Pang Y W, Duan L Z, Song D Y, et al. A modified method of genomic DNA extraction and establishment of AFLP systems in Rhus chinensis [J]. Guihaia, 2011, 31(1): 36-38. 庞雅文, 段立柱, 宋德应, 等. 盐肤木基因组DNA提取方法改 进及AFLP体系的建立 [J]. 广西植物, 2011, 31(1): 36-38.
    [24] White T J, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics [M]// Innis M A, Gelfand D H, Sininsky J J, et al. PCR Protocols: A Guide to Methods and Application. San Diego: Academic Press, 1990: 315-322.
    [25] Tamura K, Peterson D, Peterson N, et al. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods [J]. Mol Biol Evol, 2011, 28(10): 2731-2739.
    [26] Xiao L Q, Zhu H. Intra-genomic polymorphism in the internal transcribed spacer (ITS) regions of Cycas revoluta: Evidence of incomplete concerted evolution [J]. Biodiv Sci, 2009, 17(5): 476-481. 肖龙骞, 朱华. 苏铁nrDNA ITS区的序列多态性: 不完全致同 进化的证据 [J]. 生物多样性, 2009, 17(5): 476-481.
    [27] Ding X Y, Wang Z T, Xu H, et al. Database establishment of the whole rDNA ITS region of Dendrobium of “Fengdou” and authentication by analysis of their sequences [J]. Acta Pharm Sin, 2002, 37(7): 567-573. 丁小余, 王峥涛, 徐 红, 等. 枫斗类石斛rDNA ITS区的全序列 数据库及其序列分析鉴别 [J]. 药学学报, 2002, 37(7): 567-573.
    [28] Li M, Sui Z H, Yi H, et al. Cloning and sequence analysis of 5.8S rRNA and ITS region between 18S and 28S rRNA of Gracilaria lemaneiformis (Rhodophyta) [J]. J Ocean Univ China (Nat Sci), 2009, 39(1): 77-83. 李敏, 隋正红, 易恒, 等. 龙须菜5.8S rRNA和ITS区的克隆与 系统学分析 [J]. 中国海洋大学学报: 自然科学版, 2009, 39(1): 77-83.
    [29] Komatsu T, Tatsukawa K, Filippi J B, et al. Distribution of drifting seaweeds in eastern East China Sea [J]. J Mar Syst, 2007, 67(3/4): 245-252.
    [30] Komatsu T, Matsunaga D, Mikami A, et al. Abundance of drifting seaweeds in eastern East China Sea [J]. J Appl Phycol, 2008, 20(5): 801-809.
    [31] Wang W J, Wang F J, Chen S L, et al. PCR amplification and sequence analysis of ITS regions of Enteromorpha prolifera [J]. Mar Fish Res, 2008, 29(5): 124-129. 汪文俊, 王飞久, 陈松林, 等. 浒苔ITS区的扩增和分析 [J]. 海 洋水产研究, 2008, 29(5): 124-129.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

蔡一凡,张鹏,王铁杆,陈少波,谢起浪.基于5.8S rDNA-ITS序列的我国浙江沿海铜藻群体遗传多样性分析[J].热带亚热带植物学报,2013,21(2):147~154

复制
分享
文章指标
  • 点击次数:2715
  • 下载次数: 2721
  • HTML阅读次数: 0
  • 引用次数: 0
历史
  • 收稿日期:2012-08-17
  • 最后修改日期:2012-11-15
  • 录用日期:2013-01-22
  • 在线发布日期: 2013-04-03
文章二维码