基于rbcL和matK序列探讨马鞭草科部分植物的系统学位置

Systematic Position of Partial Plants of Verbenaceae Inferred from Sequences of Chloroplast rbcL and matK

摘要:

为探究适用于马鞭草科植物的DNA条形码及该类群的系统分类关系,对豆腐柴(Premna microphylla)的叶绿体基因ycf6-psbM、trnV-atpE、rbcL、trnL-F、psbM-trnD、atpB-rbcL、trnC-ycf6、trnH-psbA、rpl36-infA-rps8和核基因ITS序列进行了PCR扩增和测序,结果表明,仅rbcL、trnl-F、trnH-psbA序列的PCR扩增以及测序效果较好,而ITS不能得到明显的扩增条带,ycf6-psbM不能成功测序,其它序列存在有部分双峰或噪值高等问题。根据DNA条形码标准,rbcL序列是所有测试条码中相对最适合的。应用rbcL和matK序列对马鞭草科(Verbenaceae)豆腐柴属、牡荆属(Vitex L.)、马鞭草属(Verbena L.)和大青属(Clerodendrum L.)等4属与唇形科宝盖草属(Lamium L.)、水苏属(Stachys L.)、鼠尾草属(Salvia L.)和香科科属(Teucrium L.)等4属的分类和系统发育关系进行分析,以紫草科Lithospermum multiflorum L. 为外类群,最大简约法对2个片段的单独和联合矩阵分别构建系统发育树。豆腐柴属和大青属应从马鞭草科划入唇形科,马鞭草属仍归于马鞭草科,而牡荆属的系统学位置还需更多的证据。

Abstract:

In order to understand systematic relationship of Verbenaceae, chloroplast genes, such as ycf6-psbM, trnV-atpE, rbcL, trnL-F, psbM-trnD, atpB-rbcL, trnC-ycf6, trnH-psbA, rpl36-infA-rps8 and nrDNA ITS of Premna microphylla Trucz. were amplified by PCR and sequenced. Among these loci, rbcL, trnL-F and trnHpsbA were efficient for both PCR amplification and DNA sequencing, while other sequences don't conform to DNA barcode standard, ITS had no specific bands, ycf6-psbM could not sequence successfully, and the others had double peaks or high noisy values. However, rbcL is better than others according to DNA barcoding standard. The phylogenetic relationships among four genera from Verbenaceae, such as Premna L., Vitex L., Verbena L., and Clerodendrum L., and four genera from Labiatae, such as Lamium L., Stachys L., Salvia L., and Teucrium L., were studied by both combined and individual analyese using the chloroplast genes rbcL and matK sequences. The gene trees were constructed by maximum parsimony method with Lithospermum multiflorum as outgroup. It suggested that Premna L. and Clerodendrum L. were supposed to be moved to Labiatae from Verbenaceae and Verbena L. in Verbenaceae taxonomically, and the systematic position of Vitex L. was worthy of further study.