鸳鸯茉莉查尔酮异构酶基因(CHI)cDNA的克隆与生物信息学分析

Cloning and Sequence Analysis of Chalcone Isomerase Gene (CHI) from Brunfelsia acuminata Flowers

摘要:

采用RT-PCR 与RACE 技术克隆了鸳鸯茉莉(Brunfelsia acuminata)花瓣中查尔酮异构酶基因(CHI)的全长cDNA,GenBank 登录号为JN887637。该基因全长1051 bp,含有1 个792 bp 的开放阅读框,编码263 个氨基酸,为不稳定蛋白。对保守区功能区的分析,推导CHI 蛋白具有查尔酮超级家族的保守结构域,二级结构预测显示其主要以α 螺旋和β 折叠为主。氨基酸同源性分析表明,鸳鸯茉莉CHI 蛋白与矮牵牛(Petunia hybrida)、金花茶(Camellia nitidissima)、甜樱桃(Prunus avium)、芍药(Paeonia lactiflora)、牡丹(P. suffruticosa)、菊花(Chrysanthemum morifolium)等植物的同源性分别达到 90%、89%、84%、85%、84%、80%。因此,CHI 基因可能与鸳鸯茉莉的花色形成有关。

Abstract:

The chalcone isomerase gene (CHI) cDNA from Brunfelsia acuminata flowers was cloned by RTPCR and RACE, with GenBank accession number JN887637. The full length cDNA of CHI was 1051 bp with an open reading frame (ORF) of 792 bp, encoding an unstable protein with 263 amino acids. CHI protein from B. acuminata flower has conserved domain belonging to chalcone superfamily. It was predicted that the secondary structure of CHI protein dominated by α-coil and β-sheet. The amino acid sequence of CHI from B. acuminata flowers had homologous to those from Petunia hybrida, Camellia nitidissima, Prunus avium, Paeonia lactiflora, P. suffruticosa, Chrysanthemum morifolium for 90%, 89%, 84%, 85%, 84% and 80%, respectively. It suggested that CHI gene related to flower color formation of B. acuminata.