巴西橡胶树HbNAC1基因的克隆和表达分析

Cloning and Expression Analysis of HbNAC1 in Hevea brasiliensi

摘要:

根据EST 序列信息,利用RACE 技术从巴西橡胶树(Hevea brasiliensis)中克隆了1 个NAC 类转录因子基因HbNAC1,其cDNA 全长为1419 bp,含有完整的开放阅读框,编码309 个氨基酸。推导的氨基酸序列含有1 个NAM 结构域,具有典型的NAC 类蛋白的结构特征,与毛果杨(Populus trichocarpa)、蓖麻(Ricinus communis)、水稻(Oryza sativa)和拟南芥(Arabidopsisthaliana)中的相应氨基酸序列的同源性分别达79%、80%、57% 和60%。聚类分析表明,HbNAC1 属于NAC 家族Ⅱ亚族。半定量RT-PCR 分析表明,该基因在胶乳中的表达较丰富,经乙烯利刺激后其在胶乳中的表达量明显增加。

Abstract:

Based on EST sequences from an ethephon-induced latex SSH cDNA library of Hevea brasiliensis, a full-length cDNA, named as HbNAC1, was cloned from H. brasiliensis by RACE-PCR, encoding a NAC transcription factor. The full-length of HbNAC1 cDNA was 1419 bp with an open reading frame (ORF) of 930 bp, and encoding 309 amino acids. The deduced HbNAC1 with NAM domains had the typical characteristics of NAC family, and homologous to NACs in Populus trichocarpa, Ricinus communis, Oryza sativa and Arabidopsis thaliana of 79%, 80%, 57% and 60%, respectively. Phylogenetic analysis showed that HbNAC1 was classified into the Ⅱ subfamily of NAC family. Semi-quantitative RT-PCR analysis indicated that the expression of HbNAC1 was rich in latex, and significantly enhanced by ethephon stimulation.