福建农林大学甘蔗综合研究所,农业部甘蔗遗传改良重点开放实验室, 福建农林大学甘蔗综合研究所,福建农林大学生命科学学院,福建农林大学水稻遗传研究所,农业部甘蔗遗传改良重点开放实验室, 福建农林大学甘蔗综合研究所,农业部甘蔗遗传改良重点开放实验室, 福建农林大学甘蔗综合研究所,农业部甘蔗遗传改良重点开放实验室, 福建农林大学甘蔗综合研究所,农业部甘蔗遗传改良重点开放实验室, 福建农林大学甘蔗综合研究所
国家自然科学基金项目(30700514); 农业部2010 年引进国际先进农业科学技术“948”计划项目(2010-C21)资助
Fujian Agriculture and Forestry University, Sugarcane Institute,Key Lab of Sugarcane Genetic Improvement, MOA, P.R.China. Fujian Agriculture and Forestry University,ife Science College, Fujian Agriculture and Forestry University,Genetic Institute of Rice, Fujian Agriculture and Forestry University,Key Lab of Sugarcane Genetic Improvement, MOA, P.R.China. Fujian Agriculture and Forestry University,Key Lab of Sugarcane Genetic Improvement, MOA, P.R.China. Fujian Agriculture and Forestry University,Key Lab of Sugarcane Genetic Improvement, MOA, P.R.China. Fujian Agriculture and Forestry University,Key Lab of Sugarcane Genetic Improvement, MOA, P.R.China. Fujian Agriculture and Forestry University
对甘蔗(Saccharum officinarum L.)叶片全长cDNA 文库进行测序,获得了1 个细胞质型苹果酸脱氢酶(cMDH)基因的全长cDNA 序列,命名为Sc-cMDH。生物信息学分析表明,该基因全长1314 bp,开放阅读框为999 bp,编码332 个氨基酸。SccMDH与其他植物cMDH 的氨基酸序列同源性高达86.5%~97.0%。Sc-cMDH 包含典型的NAD+ 结合基元T11GAAGQI17 和催化基元I184WGNH188,还有相当保守的6 个半胱氨酸残基,因此推断该基因为细胞质型NAD-MDH。定量PCR 分析结果表明,该基因在甘蔗叶片和根中的表达量高于茎。
A full-length cDNA sequence of sugarcane (Saccharum officinarum L.) cytosolic malate dehydrogenase (cMDH) gene, named as Sc-cMDH, was obtained from sugarcane full-length cDNA library by EST sequencing and bioinformatics analysis. The full-length of Sc-cMDH was 1314 bp with 999 bp open reading frame, encoding 332 amino acids. Sc-cMDH shared 86.5%~97.0% of amino acids with cMDH from other 9 species. Sc-cMDH contains typical NAD+ binding motif (T11GAAGQI17) and catalytic motif (I184WGNH188). Real-time PCR analysis showed that the expression of Sc-cMDH was higher in leaves and roots than that in stems of sugarcane.
徐景升,阙友雄,颜克伟,唐唯其,许莉萍,高三基,郭晋隆,董萌,陈如凯.甘蔗细胞质型苹果酸脱氢酶基因的克隆及其表达分析[J].热带亚热带植物学报,2012,20(5):455~461
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