粤东橄榄资源核心种质取样方案的研究

Studies on Sampling Schemes of Core Collection of Canarium album L. in Eastern Guangdong, China

摘要:

为研究基于分子标记数据构建橄榄(Canarium album L.)核心种质的取样方案, 以粤东地区64份橄榄种质材料的ISSR分析结果为基础,分别利用SM和Nei & Li遗传距离,采用UPGMA聚类法进行多次聚类随机取样,比较了不同分组情况下P、S、L和G等取样策略对核心种质构建的影响。结果表明,通过比较不同样品群的多态性位点数、多态性位点百分率、观测等位基因数、有效等位基因数、Nei’s遗传多样性指数和Shannon’s信息指数等参数,最终选择根据Nei & Li法计算遗传距离,G策略取样得到的16个样品作为核心种质。该核心种质保留了初始种质25%的样品,多态性位点和多态性位点百分率保留率分别为92.93%和98.31%,观测等位基因数、有效等位基因数、Nei’s遗传多样性指数、Shannon’s信息指数的保留率分别为9.26%、102.56%、107.39%和106.29%。因此,按该方案进行取样的核心种质可以较好地代表原有种质库的遗传多样性。

Abstract:

The Effects of sampling schemes on construct method for core collection of Canarium album L. based on molecular markers data were studied on the basis of ISSR results of 64 germplasm materials of C. album from eastern Guangdong, and using UPGMA cluster method according to SM and Nei & Li genetic distance by stepwise clustering. The impact of sampling strategies, such as P, S, L and G, on the construction of core collection with different groupings was discussed. Compared with the number of polymorphic loci, percentage of polymorphic loci, observed number of alleles, effective number of alleles, Nei’s genetic diversity index and Shannon’s information index of different sample groups, 16 samples were finally chosen as the core collection according to genetic proportion strategy based on Nei & Li genetic distance. The core collection retained 25% of germplasm of the initial samples, and the retention ratio of the number of polymorphic loci and percentage of polymorphic loci was 92.93% and 98.31%, respectively, and the observed number of alleles, effective number of alleles, Nei’s genetic diversity index and Shannon’s information index reached 99.26%, 102.56%, 107.39% and 106.29%, respectively. It suggested that the core collection constructed by this method could goodly represent the genetic diversity of initial germplasm bank.