Gibberella fujikuroi侵染果蔗叶片病程相关蛋白编码基因的表达分析

The Expression Analysis of Pathogenesis-related Protein Encoding Genes in Chewing Cane Leaves Infected by Gibberella fujikuroi

摘要:

利用实时荧光定量RT-PCR,用梢腐病病原菌(Gibberella fujikuroi)侵染不同果蔗品种叶片,对病程相关蛋白编码基因SoSOD、SoCHIT、SoPOD与SoTPS6P的转录水平进行了分析。结果表明,果蔗的SoSOD、SoCHIT、SoPOD与SoTPS6P受到梢腐病病原菌的诱导表达,丰城紫皮、白鳝、福安与拔地拉果蔗叶片中它们的表达量较高,而在温岭、宁德与歪干担叶片中的表达量较低。这说明这些病程相关蛋白编码基因表达水平与不同果蔗品种的梢腐病抗病性存在一定的关联。

Abstract:

The pathogenesis-related protein encoding genes were cloned from sugarcane (Saccharum officenarum) leaves, named as SoSOD、SoCHIT、SoPOD and SoTPS6P. Their expression in S. officenarum leaves infected by Gibberella fujikuroi was studied by real-time quantitative RT-PCR. The results showed that the expression of SoSOD、SoCHIT、SoPOD and SoTPS6P genes could be induced in chewing cane leaves after and the expression levels in leaves of different chewing cane cultivars were different. The expression levels of these genes in cultivars ‘Fengchengzipi’, ‘Baishang’, ‘Fuan’and ‘adila’leaves were higher than those in cultivars ‘Wenlin’, ‘Ninde’and ‘Waigandan’. These indicated that the expression levels of SoSOD、SoCHIT、SoPOD and SoTPS6P associated with the resistance of different chewing cane cultivars to G. fujikuroi.