板栗和锥栗同域居群的空间遗传结构
作者:
基金项目:

国家自然科学基金项目(30770219);中国科学院重要方向性项目(KSCX2-YW-N-061)资助


Spatial Genetic Structure of A Sympatric Population of Castanea mollissima and Castanea henryi
Author:
  • 摘要
  • | |
  • 访问统计
  • |
  • 参考文献 [34]
  • |
  • 相似文献
  • |
  • 引证文献
  • | |
  • 文章评论
    摘要:

    采用微卫星技术研究了板栗(Castanea mollissima)和锥栗(C. henryi)同域分布居群的空间遗传结构, 探讨了它们的遗传变异空间分布特征及其形成机制。结果表明,采用的7个微卫星在两物种中共扩增出173个等位基因,两物种都具有较高的遗传多样性且种间遗传分化水平较低(FST=0.051), 但空间自相关分析揭示板栗和锥栗在同域居群中具有不同的空间遗传结构,锥栗在100 m内有空间遗传结构,而板栗没有;同时,基于亲缘关系系数FijSp统计值也显示锥栗具有比板栗更强的空间遗传结构(板栗的Sp=0.002;锥栗的Sp=0.018)。居群遗传变异的空间结构是其传粉和种子散播及生境共同作用的结果,其中种子的近距离散播和居群密度可能是主要的因素。板栗和锥栗居群在小尺度上空间遗传结构的差异可能反映了它们种子的大小及扩散过程的差异。

    Abstract:

    The spatial genetic structure (SGS) is an important part of evolutionary ecological and ecological genetic processes in natural populations of plants. The spatial distribution patterns of genetic variation of two closely related sympatric Castanea species, C. mollissima and C. henryi, were investigated using seven microsatellite loci. A total of 173 alleles were detected in the two species. According to the isolation by distance model, Sp-statistics were calculated based on Moran's I spatial autocorrelation coefficient and Fij kinship coefficient for the two species. The results showed that there were high polymorphism in each species with low genetic differentiation ( FST=0.051). However, the two species displayed significant difference of SGS in this sympatric population. Spatial genetic structure was detected in C. henryi up to 100 m, while that was not observed in C. mollissima. Moreover, the Sp-statistic values based on Fij, were 0.002 for C. mollissima and 0.018 for C. henryi. These results further supported that C. henryi had stronger spatial genetic structure than C. mollissima. The difference of SGS in C. mollissima and C. henryi can be explained by different seed characteristics, as C. mollissima was charactertized by three nuts per cupule while there was only one nuts per cupule for C. henryi. Therefore, the long-distance seed dispersal via gravity and animals was much more favored for C. mollissima than C. henryi, which reduced different SGS between the two species.

    参考文献
    [1] Johnson G P. Revision of Castanea sect. Balanocastanon (Fagaceae) [J]. J Arn Arb, 1988, 69(1): 25-49.
    [2] Huang C C(黄成就), Chang Y T(张永田), Hsu Y C(徐永椿), et al. Fagaceae [M] // Chun W Y(陈焕镛), Huang C C(黄成就). Flora Reipublicae Popularis Sinicae Tomus 22. Beijing: Science Press, 1998: 8-13.(in Chinese)
    [3] Hamrick J L, Godt Mary J W, Hamrick J L, et al. Conservation Genetics of Endemic Species [M]. New York: Chapman and Hall, 1996: 281-304.
    [4] Sokal R R, Oden N L. Spatial autocorrelation in biology: 1. Methodology [J]. Biol J Soc, 1978, 10(2): 199-228.
    [5] Sokal R R, Oden N L. Spatial autocorrelation in biology: 2. Some biological implications and four applications of evolutionary and ecological interest [J]. Biol J Soc, 1978, 10(2): 229-249.
    [6] Brown A H D, Clegg M T, Kahler A L, et al. Spatial Patterns of Genetic Variation within Plant Populations [M]. MA: Sinauer Associates, 1990: 229-253.
    [7] He T H(何田华), Yang J(杨继), Rao G Y(饶广远). Spatial autocorrelation analysis of plant population genetic variation [J]. Chin Bull Bot(植物学通报), 1999, 16(6): 636-641.(in Chinese)
    [8] Wang Y(王英), Kang M(康明), Huang H W(黄宏文). Subpopulation genetic structure in a panmictic population as revealed by molecular markers: A case study of Castanea sequinii using SSR markers [J]. J Plant Ecol(植物生态学报), 2006, 30(1): 147-156.(in Chinese)
    [9] Cottrell J C, Munro R C, Tabbener H E, et al. Comparison of fine-scale genetic structure using nuclear microsatellites within two British oakwoods differing in population history [J]. For Ecol Manag, 2003, 176(1/2/3): 287-303.
    [10] Brunsfeld S J, Soltis D E, Soltis P S. Evolutionary patterns and processes in Salix sect. Longifoliae : Evidence from chloroplast DNA [J]. Syst Bot, 1992, 17(2): 239-256.
    [11] Marinoni D, Akkak A, Bounous G, et al. Development and characterization of microsatellite markers in Castanea sativa Mill. [J]. Mol Breed, 2003, 11(2): 127-136.
    [12] Yamamoto T, Tahaka T, Kotobuki K, et al. Characterization of simple sequence repeats in Japanese chestnut [J]. J Hort Sci Biotechn, 2003, 78(2): 197-203.
    [13] Tian H(田华), Kang M(康明), Li L(李丽), et al. Genetic diversity in natural populations of Castanea mollissima inferred from nuclear SSR markers [J]. Biodiv Sci(生物多样性), 2009, 17(3): 296-302.(in Chinese)
    [14] Peakall R, Smouse P E. GENALEX 6: Genetic analysis in Excel. Poulation genetic software for teaching and research [J]. Mol Ecol Notes, 2006, 6(1): 288-295.
    [15] Wright S. Coefficients of inbreeding and relationship [J]. Amer Nat, 1922, 56(645): 330-338.
    [16] Geburek T. Are gene randomly distributed over space in mature populations of sugar maple (Acer saccharum Marsh.) [J]. Ann Bot, 1993, 71(3): 217-222.
    [17] Vekemans X, Hardy O J. New insights from fine-scale spatial genetic structure analyses in plant populations [J]. Mol Ecol, 2004, 13(4): 921-935.
    [18] Hardy O J, Maggia L, Bandou E, et al. Fine-scale genetic structure and gene dispersal inferences in 10 Neotropical tree species [J]. Mol Ecol, 2006, 15(2): 559-571.
    [19] Hardy O J, Vekemans X. Spagedi: A versatile computer program to analyse spatial genetic structure at the individual or population levels [J]. Mol Ecol Notes, 2002, 2(4): 618-620.
    [20] Loiselle B A, Sork V L, Nason J, et al. Spatial genetic structure of a tropical understory shrub, Psychotria officinalis (Rubiaceae) [J]. Amer J Bot, 1995, 82(11): 1420-1425.
    [21] Lang P(郎萍), Huang H W(黄宏文). Genetic diversity and geographic variation in natural populations of the endemic Castanea species in China [J]. Acta Bot Sin(植物学报), 1999, 41(6): 651-657.(in Chinese)
    [22] Li Z Z(李作洲), Lang P(郎萍), Huang H W(黄宏文). Spatial structure of allozyme frequencies in Castanea mollissima Bl. [J]. J Wuhan Bot Res(武汉植物学研究), 2002, 20(3): 165-170.(in Chinese)
    [23] Liu Y(刘莹), Ning Z L(宁祖林), Wang J(王静), et al. Study on leaf phenotypic variation of a sympatric population of Castanea mollissima and C. henryi [J]. J Wuhan Bot Res(武汉植物学研究), 2009, 27(5): 480-488.(in Chinese)
    [24] Muir G, Schlotterer C. Evidence for shared ancestral polymorphism rather than recurrent gene flow at microsatellite loci differentiating two hybridizing oaks (Quercus spp.) [J]. Mol Ecol, 2005, 14(2): 549-561.
    [25] Soto A, Lorenzo Z, Gil L. Differences in fine-scale genetic structure and dispersal in Quercus ilex L. and Q. suber L.: Consequences for regeneration of Mediterranean open woods [J]. Heredity, 2007, 99(6): 601-607.
    [26] Rousset F. Genetic differentiation and estimation of gene flow from F-statistics under isolation by distance [J]. Genetics, 1997, 145(4): 1219-1228.
    [27] Valbuena-Carabaa M, Gonzalez-Martinez S C, Hardy O J, et al. Fine-scale spatial genetic structure in mixed oak stands with different levels of hybridization [J]. Mol Ecol, 2007, 16(6): 1207-1219.
    [28] Kalisz S, Nason J D, Hanzawa F A, et al. Spatial population genetic structure in Trillium grandiflorum: The roles of dispersal, mating history and selection [J]. Evolution, 2001, 55(8): 560-568.
    [29] Jü T Z(巨天珍). A quantitation study of the interspecific association of Quercus aliena var. acuteserrata community in Xiaolongshan Mountain of Tianshui [J]. Acta Bot Boreal-Occid Sin(西北植物学报), 1995, 15(3): 250-253.(in Chinese)
    [30] Xiao Z S(肖治术), Zhang Z B(张知彬). Hoarding behavior of rodents and plant seed dispersal [J]. Acta Theriol Sin(兽类学报), 2004, 24(2): 61-67.(in Chinese)
    [31] El-Kassaby Y A, Jaquish B. Population density and mating pattern in Western Larch [J]. Heredity, 1996, 87(6): 438-443.
    [32] Dyer R J, Sork V L. Pollen pool heterogeneity in shortleaf pine, Pinus echinata Mill. [J]. Mol Ecol, 2001, 10(4): 859-866.
    [33] Sousa V A, Hattemer H H. Pollen dispersal and gene flow by pollen in Araucaria angustifolia [J]. Botany, 2003, 51(3): 309-317.
    [34] Manel S, Sehwartz M K, Luikart G, et al. Landscape genetics: Combining landscape ecology and population genetics [J]. Trend Ecol Evol, 2003, 18(4): 189-197.
    相似文献
    引证文献
引用本文

朱蕾,康明.板栗和锥栗同域居群的空间遗传结构[J].热带亚热带植物学报,2012,20(1):1~7

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2011-05-09
  • 最后修改日期:2011-06-02
  • 录用日期:2011-06-02
  • 在线发布日期: 2012-01-11
文章二维码