海南蕊木枝叶生物活性成分研究

Bioactive Constituents from Twigs and Leaves of Kopsia hainanensis

摘要:

从海南蕊木(Kopsia hainanensis Tsiang)枝叶的乙醇提取物中分离得到7个化合物,通过波谱分析(NMR、MS、IR等),鉴定其结构分别为:蕊木宁(1)、土波台文碱(2)、二氢脱氢二松柏基醇(3)、 β-香树脂醇(4)、熊果酸(5)、 2α,3β-二羟基熊果酸(6)和 β-谷甾醇(7)。其中化合物3~7为首次从该植物中分离得到。用滤纸片琼脂扩散法检测表明,化合物1~3和5对耐甲氧西林金黄色葡萄球菌(MRSA)生长有抑制作用;化合物2对金黄色葡萄球菌生长有抑制作用;化合物5对慢性髓原白血病细胞(K562)、人胃癌细胞(SGC-7901)和人肝癌细胞(SMMC-7721)增殖均具有细胞毒活性。

Abstract:

Seven compounds were isolated from the ethanolic extract of twigs and leaves of Kopsia hainanensis Tsiang. On the basis of spectral data (NMR, MS, IR, etc.), their structures were identified as kopsinine (1), tubotaiwine (2), dihydrodehydrodiconiferyl alcohol (3), β-amyrin (4), ursolic acid (5), 2 α,3β-dihydroxyursolic acid (6), and β-sitosterol (7). Compounds 3-7 were isolated from K. hainanensis for the first time. Antibacterial activities assayed by paper disk diffusion method, the result showed that compounds 1-3, and 5 had inhibitory activities against methicillin-resistant Staphylococcus aureus (MRSA), and compound 2 exhibits inhibitory effect on Staphylococcus aureus. Cytotoxic activity assay demonstrated that compound 5 showed cytotoxic activities towards chronic myelogenous leukemia (K562), human gastric carcinoma (SGC-7901), and human hepatoma (SMMC-7721) cell lines.