农杆菌介导的番茄遗传转化的研究进展

Progresses in Agrobacterium-mediated Transformation of Tomato

摘要:

番茄(Lycopersicon esculentum)遗传转化体系对其功能基因的研究和基因工程育种有重要影响,对农杆菌(Agrobacterium tumefaciens)介导的番茄遗传转化的研究进展进行了综述,主要包括影响番茄遗传转化效率的几个因素,如番茄的基因型、外植体状态、预培养和侵染过程、分化培养基中的激素和抗生素配比以及不同的外源基因等问题。并对今后的番茄遗传转化研究进行了展望。

Abstract:

The progresses in Agrobacterium-mediated transformation of tomato (Lycopersicon esculentum) was reviewed. Several factors were summarized, such as tomato genotypes, explants, the processes of preculture and infection, the ratio of plant growth regulators and antibiotics in regeneration medium, and different target gene. The tomato genetic transformation in future was prospected.