首页 > 过刊浏览>2011年第19卷第5期 >407-411. DOI:10.3969/j.issn.1005-3395.2011.05.003
PDF HTML阅读 XML下载 导出引用 引用提醒
水稻永绿色基因超表达和突变对其叶片氮碳代谢若干指标的影响
作者:
基金项目:

国家自然科学基金项目(30770174); 863计划项目(2006AA10Z163)资助


Effects of Stay-green Rice Gene (SGR) Over-expression and Mutation on Some Indexes Related with Carbon and Nitrogen Metabolism in Rice Leaves
Author:
  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献 [24]
    • |
  • 相似文献 [20]
    • | | |
  • 文章评论
    摘要:

    通过水培实验,研究了水稻永绿色(Stay-green rice,SGR)基因超表达和突变对叶片氮碳代谢的影响。结果表明,在正常生长条件下,SGR基因超表达降低了水稻叶片可溶性蛋白、叶绿素及淀粉的含量,但可溶性糖和游离氨基酸含量增加,并提高了谷氨酰胺合成酶(GS)活性和谷氨酸合成酶(GOGAT)活性;SGR基因突变增加了水稻叶片淀粉和可溶性蛋白质含量,并提高了硝酸还原酶(NR)活性。在缺氮条件下,SGR基因超表达与野生型叶片各生理指标的变化趋势一致,但是SGR基因突变体叶片中淀粉含量的变化趋势与野生型及SGR基因超表达的不一致。这说明SGR蛋白水平的变化在一定程度上影响了水稻叶片的氮碳代谢。

    Abstract:

    The effects of Stay-green rice gene (SGR) over-expression and mutation on carbon and nitrogen metabolism in rice (Oryza sativa L.) leaves were studied by using hydroponic culture. The results showed that SGR over-expression reduced contents of soluble protein, chlorophyll and starch, but the contents of soluble sugar and free amino acids increased, as well as the activities of glutamine synthetase (GS) and glutamate synthetase (GOGAT) in rice leaves under normal growth condition. Whereas, SGR mutation increased the contents of starch and soluble protein and improved nitrate reductase (NR) activity. Under nitrogen deficiency, the variation trend of physiological parameters was consistent among the three types of rice except the starch content in SGR mutant rice leaves. These suggested that the changes in SGR level could influence the carbon and nitrogen metabolism in rice leaves.

    参考文献
    [1] Jiang H W, Li M R, Liang N T, et al. Molecular cloning and function analysis of the stay green gene in rice [J]. Plant J, 2007, 52(2): 197-209.
    [2] Thomas H, Howarth C J. Five ways to stay green [J]. J Exp Bot, 2000, 51(1): 329-337.
    [3] Franoise D V, Anne K, Wenner M. Cellular biology of nitrogen metabolism and signaling [J]. Plant Cell Monogr, 2010, 17: 145-172.
    [4] Pourtau N, Mares M, Purdy S, et al. Interactions of abscisic acid and sugar signaling in the regulation of leaf senescence [J]. Planta, 2004, 219(5): 765-772.
    [5] Kato Y, Yamamoto Y, Murakami S, et al. Post-translational regulation of CND41 protease activity in senescent tobacco leaves [J]. Planta, 2005, 222(4): 643-651.
    [6] 戴忠民. 氮素代谢对小麦生理特性的影响研究进展 [J]. 河南农业科学, 2008(7): 10-13.
    [7] Lam H M, Coschigano K T, Oliveiva I C. The molecular genetics of nitrogen assimilation into amino acids in higher plants [J]. Annu Rev Plant Physiol Plant Mol Biol, 1996, 47: 569-593.
    [8] Jin J Y(金继运), He P(何萍). Effect of N and K nutrition on post metabolism of carbon and nitrogen and grain weight formation in maize [J]. Sci Agri Sin(中国农业科学), 1999, 32(4): 55-62.(in Chinese)
    [9] Yoshida S, Forno D A, Cock J H, et al. Laboratory Manual for Physiological Studies of Rice [M]. 3rd ed. Manila, Philippines: International Rice Research Institute, 1976: 61-64.
    [10] Kamachi K, Yamaya T, Mae T, et al. A role for glutamine synthetase in the remobilization of leaf nitrogen during natural senescence in rice leaves [J]. Plant Physiol, 1991, 96(2): 411-417.
    [11] Schelbert S, Aubry S, Burla B, et al. Pheophytin pheophorbide hydrolase (Pheophytinase) is involved in chlorophyll breakdown during leaf senescence in Arabidopsis [J]. Plant Cell, 2009, 21(3): 767-785.
    [12] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [J]. Anal Biochem, 1976, 72(1/2): 248-254.
    [13] Agüera E, Ruano D, Cabello P, et al. Impact of atmospheric CO2 on growth, photosynthesis and nitrogen metabolism in cucumber (Cucumis sativus L.) plants [J]. Plant Physiol, 2006, 163(8): 809-817.
    [14] Rosen H. A modified ninhydrin colorimetric analysis for amino acids [J]. Arch Biochem Biophys, 1957, 67(1): 10-15.
    [15] Li H S(李合生). Principles and Techniques of Plant Physio-logical Biochemical Experiment [M]. Beijing: Higher Education Press, 2000: 125-127.(in Chinese)
    [16] Wang X C(王小纯), Xiong S P(熊淑萍), Ma X M(马新明), et al. Effect of different nitrogen forms on key enzyme activity involved in nitrogen metabolism and grain protein content in specialty wheat cultivars [J]. Acta Ecol Sin(生态学报), 2005, 25(4): 802-807.(in Chinese)
    [17] Thomas L, Laure G, Stéphanie B M and et al. Enzymatic and metabolic diagnostic of nitrogen deficiency in Arabidopsis thaliana Wassileskija accession [J]. Plant Cell Physiol, 2008, 49(7): 1056-1065.
    [18] Tercé-Laforgue T, Mck G, Hirel B. New insights towards the function of glutamate dehydrogenase revealed during source-sink transition of tobacco (Nicotiana tabacum) plants grown under different nitrogen regimes [J]. Physiol Plant, 2004, 120(2): 220-228.
    [19] Park S Y, Yu J W, Park J S, et al. The senescence-induced staygreen protein regulates chlorophyll degradation [J]. Plant Cell, 2007, 19(5): 1649-1664.
    [20] Evans R J. Nitrogen and photosynthesis in the flag leaf of wheat (Triticum aestivum L.) [J]. Plant Physiol, 1983, 72(2): 297-302.
    [21] Dian W M, Jiang H W, Chen Q S, et al. Cloning and charac-terization of the granule-bound starch synthase II gene in rice: Gene expression is regulated by the nitrogen level, sugar and circadian rhythm [J]. Planta, 2003, 218(2): 261-268.
    [22] Wu W H(武维华). Plant Physiology [M]. Beijing: Science Press, 2003: 90-91.(in Chinese)
    [23] Vincentz M, Moureaux T, Leydecker M T, et al. Regulation of nitrate and nitrite reductase expression in Nicotiana plumbaginifolia leaves by nitrogen and carbon metabolites [J]. Plant J, 1993, 3(2): 315-324.
    [24] Paula M M, Ligia M L, Isabel M S, et al. Expression of the plastid located glutamine synthetase of Medicago truncatula [J]. Plant Physiol, 2003, 132(1): 390-399.
    引证文献
    网友评论
    网友评论
    分享到微博
    发 布
引用本文

戎红,李美茹,陈雅平,吴国江,姜华武.水稻永绿色基因超表达和突变对其叶片氮碳代谢若干指标的影响[J].热带亚热带植物学报,2011,19(5):407~411

复制
分享
文章指标
  • 点击次数:3258
  • 下载次数: 2279
  • HTML阅读次数: 0
  • 引用次数: 0
历史
  • 收稿日期:2011-01-05
  • 最后修改日期:2011-04-25
  • 录用日期:2011-06-20
  • 在线发布日期: 2011-09-27
文章二维码
您是第16727902 位访问者
主管单位：中国科学院
主办单位：中国科学院华南植物园 广东省植物学会
地址：广州市天河区兴科路723号 中国科学院华南植物园 《热带亚热带植物学报》编辑部
电话：86 (0)20-3725 2514
热带亚热带植物学报 ® 2025 版权所有