水稻永绿色基因突变和超表达对其叶片类囊体蛋白降解的影响

Effects of Stay-green Rice Gene (SGR) Mutation and Overexpression on the Protein Degradation in Thylakoid of Rice Leaves

摘要:

应用蛋白质免疫杂交技术分析了永绿色基因(Stay-green Rice,SGR)突变和超表达对水稻(Oryza sativa)叶片类囊体蛋白质降解的影响。结果表明, 在正常生长条件下,SGR超表达降低了光系统Ⅱ(PSⅡ)、光系统Ⅰ(PSⅠ)和电子传递链等的蛋白质含量。暗诱导衰老处理时,SGR突变延缓了PSI和PSII的蛋白降解,对电子传递链相关蛋白的降解无影响。缺氮胁迫下,SGR超表达加快了PSII 和PSI的蛋白降解,SGR突变延缓了类囊体蛋白的降解。因此,SGR超表达能促进类囊体PSI、PSII和光合电子传递链蛋白质的降解。

Abstract:

The effects of stay-green rice gene (SGR) mutation and overexpression on thylakoid protein degradation in rice leaves were studied by using protein immunoblot method. The results showed that the overexpression of SGR reduced the contents of protein in photosystem II (PSII), as well as PSI and electron transport chain under normal growth condition. During the dark-induced senescence, SGR mutation delayed the degradation of PSII and PSI proteins, but proteins in the electron transport chain had no changes. Under nitrogen deficiency stress, the overexpression of SGR promoted the thylakoid protein degradation, whereas SGR mutation delayed the thylakoid protein degradation. Therefore, the overexpression of SGR could promote the degradation of thylakoid protein in photosystem I and II, and electron transport chain.