荔枝果皮总蛋白质提取及双向电泳体系的建立

Extraction of Total Protein from Litchi Pericarps and Establishment of Two-dimensional Electrophoresis

摘要:

用TCA-丙酮、丙酮和酚3种方法提取荔枝(Litchi chinensis Sonn.)果皮的总蛋白质，比较了蛋白产量、单向SDS-PAGE和双向电泳等方面的差异，并对双向电泳体系进行探索。结果表明：酚抽提法最佳，提取的总蛋白得率最高，蛋白在单向SDS-PAGE中形成条带数目最多，最清晰；经双向电泳分离用银染显色，可检测约870个蛋白点，图谱分辨率较好，蛋白点清晰，主要分布在pH 4～8，分子量约为15.0～85.0 kD，可有效解析荔枝果皮的蛋白质组。

Abstract:

The total protein was extracted from Lichi chinensis pericarp by using three methods, such as trichloroacetic acid (TCA)-acetone, acetone and phenol. The results showed that the total protein yield was the highest by phenol extraction method, and protein bands were the most and clear in one dimentional SDS-PAGE. About 870 clear protein spots were detected by two-dimensional electrophoresis (2-DE) and silver staining with isoelectric points at pH 4~8, and relative molecular weight of protein ranged from 15.0 to 85.0 kD.