非生物胁迫下水稻OsMATE基因表达分析

EExpression Analysis of OsMATE in Rice under Abiotic Stresses

摘要:

从水稻(Oryza sativa L.)叶片中克隆到与高粱(Sorghum bicolor) SbMATE最相似的同源基因OsMATE，利用半定量RT-PCR分析了OsMATE在水稻不同组织、不同非生物胁迫以及抗铝和铝敏感水稻品种中的表达。结果表明，OsMATE在水稻根和叶中表达非常低，叶鞘中几乎没有表达；铝、镉、砷、盐、铁、PEG6000、百草枯和ABA等非生物胁迫都可以诱导水稻根OsMATE的大量表达，而在水稻叶中，只有砷、盐和PEG6000可以少量诱导OsMATE的表达；并且铝毒胁迫下抗铝品种根中OsMATE的表达明显高于铝敏感品种。这说明非生物胁迫下OsMATE在水稻抗逆中可能发挥重要作用。

Abstract:

OsMATE, the closest homology of sorghum (Sorghum bicolor) SbMATE, was cloned by PCR technique from rice (Oryza sativa) leaves, and then its expression pattern was analysed in different tissues and under abiotic stresses by semi-quantitative RT-PCR. The result showed that OsMATE expressed lowly in rice roots and leaves, and almost none in leaf sheaths. OsMATE expressed strongly in rice roots induced by abiotic stresses, such as aluminum (Al), cadmium, arsenic, salt, iron, PEG6000, paraquat and ABA, while little expressed in leaves by arsenic, salt and PEG6000 stress. The expression of OsMATE in roots of the Al-resistant ‘XN1’ was more than that in the Al-susceptible ‘XX2’ under Al stress. It suggested that OsMATE could play an important role in stress resistance of rice under abiotic stresses.