合成甘蓝型油菜中双隐性雄性核不育材料的发现及遗传规律分析

Finding and Genetic Analyzing of Double Recessive Genic Male Sterile Materials in Resynthesized Brassica napus

摘要:

从甘蓝型油菜与白菜型油菜的种间杂交获得的甘蓝型油菜(Brassica napus)中发现了雄性不育单株，兄妹交株系和不育株与甘蓝型油菜常规杂交F1和F2株系的育性分离分析表明，该不育材料属于双隐性雄性核不育类型。利用育性分离株系的可育株自交和可育株与不育株间兄妹交等方法筛选出7个纯合可育株系，等位测验表明这7个纯合可育株系(B1~B7)中存在两种基因型：Ms1Ms1ms2ms2和ms1ms1Ms2Ms2。该材料对油菜核不育基因定位和杂种优势利用研究有重要意义。

Abstract:

A male sterile line of resynthsizing Brassica napus was discovered in the interspecific crossing between Brassica napus and B. rapa. The fertility segregation in brother-sister mating (BSM) lines and the hybrids F1, F2 lines from male sterile lines and B. napus cultivars were studied, which showed that the male sterile materialswere double recessive genic male sterile. Using self-crossing and test crossing of different lines in fertility segregated lines, 7 homozygous fertile lines (B1~B7) were screened out, and two genotypes (Ms1Ms1ms2ms2 and ms1ms1Ms2Ms2) were identified. These male sterile materials would play an important role in heterosis utilization and male sterile gene mapping.