石硖龙眼未成熟种子种胚的萌发及成苗的研究

Studies on Seed Germination and Seedling of Immature Seeds and Embryos of Longan ‘Shixia’

摘要:

以花后82~87 d的石硖龙眼(Dimocarpus longan Lour. ‘Shixia’)未成熟果实在4℃贮藏24 h，研究种子与种胚的萌发与成苗情况。结果表明，河沙直播未成熟胚的最终成苗率达到80%，远高于离体培养未成熟种子的最终成苗率(12.63%～44.03%)；虽然4℃的低温贮藏略为推迟了河沙直播未成熟胚的萌发时间，但对最终成苗率及幼苗生长的影响不大，这对龙眼杂交育种中未成熟种子种胚的挽救具有较好的应用价值。

Abstract:

The germination and seedling of immature seeds and embryos after anthesis 82~87 d of longan (Dimocarpus longan Lour. ‘Shixia’) were studied. The results showed that the germination and seedling rates of immature embryos sowed directly in clean sand were better than those of in vitro cultured immature seeds. Furthermore, although the germination time of immature embryos stored at 4℃ for 24 hours was slightly delayed in sand, there was no obvious inhibition effect on their germination rate and seedling growth. These results indicated that sowing directly in clean sand was a good way for immature seed and/or embryo rescue in sexual hybridization breeding of longan.