培养条件及贮藏温度和时间对木麻黄花粉萌发率的影响

Effects of Storage Conditions on Pollen Germination of Casuarina cunninghamiana

摘要:

用离体培养的方法研究了不同蔗糖、硼酸浓度，以及不同贮藏温度和贮藏时间对木麻黄(Casuarina)花粉萌发的影响。结果表明，15%的蔗糖是木麻黄花粉萌发的最佳浓度；在15%蔗糖培养基上添加硼酸显著促进木麻黄花粉萌发，250 mg kg-1硼酸是木麻黄花粉萌发的最佳浓度；添加了琼脂的固体培养基更有利于木麻黄花粉萌发；在常温下木麻黄生活力丧失很快，但低温下花粉的萌发力可保持较长时间。

Abstract:

The effects of storage conditions on pollen germination of Casuarina cunninghamiana Miq. in vitro were studied. The results showed that the optimum concentrations of sucrose and boric acid on pollen germination of C. cunninghamiana Miq. were 15% and 250 mg kg-1, respectively. The solid medium supplemented with agar was beneficial to pollen germination. Pollen at low temperature could maintain germination ability for long time.