坪山柚上胚轴离体再生体系的建立

Plant Regeneration in vitro from Epicotyl of Citrus grandis Osbeck ‘Pingshanyou’

摘要:

以坪山柚(Citrus grandis Osbeck ‘Pingshanyou’)无菌苗的上胚轴为外植体，研究了其离体培养和植株再生技术。结果表明：坪山柚上胚轴的形态学上部出芽能力最强，诱导不定芽的最佳培养基为：MS+6-BA 1.0 mg L-1+TDZ 0.05 mg L-1+NAA 0.2 mg L-1+GA3 1.0 mg L-1+蔗糖40 g L-1，培养4周后不定芽诱导率为100%，平均不定芽数为8.35；最适生根培养基为1/2MS+NAA 1.5 mg L-1，生根率达88.7%, 平均生根6.4条；生根试管苗移栽30 d后成活率达90%。

Abstract:

The plant regeneration in vitro was studied using epicotyl segments of Citrus grandis Osbeck ‘Pingshanyou’ as explants. The results showed that the optimum explants were the upper segments of epicotyl, the optimum medium for adventitious shoots induction was MS+6-BA 1.0 mg L-1+TDZ 0.05 mg L-1+NAA 0.2 mg L-1+GA3 1.0 mg L-1+ sucrose 40 g L-1, the induction rate was 100% and the average adventitious shoots reached 8.35 after cultured for 4 weeks. The optimum medium for rooting was 1/2MS+NAA 1.5 mg L-1, the rooting and the average roots were 88.7% and 6.4, respectively. The plantlet survival was 90% after transplanted for 30 days.