Abstract:Five flavones were isolated from the ethanol extraction of the stem of Aquilaria sinensis. On the basis of spectral analysis, their structures were elucidated as: apigenin-7,4′-dimethylethers (1), 5-hydroxyl-7,3′,4′-trimethoxyflavone (2), 7,4′-dimethyl -luteolin (3), genkwanin (4) and 4′,5-dihydroxy-3′,7-dimethoxyflavone (5). All the compounds were isolated from the stem of this species for the first time.