龙眼花芽ANS基因的克隆与原核表达

Cloning and Prokaryotic Expression of Longan ANS Gene

摘要:

运用蛋白质组学比较龙眼(Dimocarpus longan Lour.)正常成花和成花逆转花芽的蛋白质组变化，结果表明，ANS蛋白(anthocyanidin synthase)在龙眼成花逆转花芽中下调表达。应用 RACE方法克隆ANS蛋白的全长cDNA，长度为1477 bp，包括1个1071 bp的开放阅读框，编码357 bp的氨基酸序列，GenBank的登录号为FJ479616(GI：218202927)。将ANS在大肠杆菌中表达，获得1个约46 kD的外源蛋白。这说明ANS蛋白在龙眼成花逆转过程中起作用。

关键词:龙眼;成花逆转;差异蛋白;ANS;克隆;表达

Abstract:

The changes in proteome of normal flower and floral reversion buds in longan (Dimocarpus longan Lour.) were studied by using Proteomics method. The results showed that ANS (anthocyanidin synthase) protein was found down-regulated expression in floral reversion buds. ANS cDNA was obtained (GenBank access number: FJ479616) by using RACE method, its full length was 1477 bp, with a 1071 bp open read frame. ANS gene was expressed in E. coli, and a 46 kD heterologous protein was obtained. It suggested that ANS protein play a role in floral reversion in longan.

Key words:Longan; Floral reversion; Differential protein; ANS; Clone; Expression