团花树形成层扩展蛋白基因cDNA的克隆和序列分析

Cloning and Sequence Analysis of Expansin gene from Cambium of Anthocephalus chinensis

摘要:

以团花树(Anthocephalus chinensis)枝条形成层的cDNA为模板，参照α-Expansin基因(EXPA)序列设计兼并引物，进行RT-PCR。以得到的扩增产物为基础，采用RACE技术得到扩展蛋白基因全长cDNA，命名为AcEXP1。AcEXP1全长979 bp(GenBank注册号为FJ417847)，开放阅读框为777 bp，编码258个氨基酸，并具有Expansin特有的保守序列和结构域。经过比对分析，该基因编码的氨基酸序列与毛白杨、欧洲山杨×美洲山杨、樱桃和矮牵牛的α-Expansin基因编码的氨基酸同源性分别为85%、85%、84%和83%。这为研究EXP基因与团花树木质部生长速度和材质的关系提供了基础。

Abstract:

Using the cDNA from the branch cambium of Anthocephalus chinensis as the template, the degenerate primers by reference to the α-Expansin gene (EXPA) sequences in other plants were constructed for RT-PCR. Taking the production of the amplification as the starting information, The full-length cDNA of expansin gene was obtained by RACE, named as AcEXP1. It was 979 bp in length (GenBank accession No. FJ417847) with 777 bp ORF, encoding 258 amino acid, which had the typical conserved sequence and domain of Expansin. There were 85%, 85%, 84%, 83% homology with Populua tomentosa, Populus tremula×P. tremuloides, Prunus cerasus, Petunia×hybrida, respectively. It provided the foundation for studying the relationships between EXP gene and growth rate, wood quality of the xylem of Anthocephalus chinensis.