基因枪法获得GAI转基因橡胶植株的研究

Obtaining the GAI Transgenic Plants of Hevea brasiliensis by Particle Bombardment

摘要:

将含有Camv35S启动子、卡那霉素抗性基因和 GUS 报告基因，目的基因为GAI 基因的转化载体质粒 pBI121，通过基因枪轰击巴西橡胶树(Hevea brasiliensis Muell.-Arg.)花药愈伤组织，50 mg L-1卡那霉素的继代培养基进行抗性筛选。获得了抗性再生植株，经过PCR、Southern检测结果表明：GAI基因已经成功转入橡胶树基因组中。

Abstract:

The vector pBI121 particle, which contains Camv35S promoter, kanamycin resistance gene, GUS reporter gene and GAI gene, was transfered into the Hevea brasiliensis anther callus by particle bombardment. The regenerated plantlets were obtained in the subculture media supplemented with kanamycin of 50 mg L-1. PCR and Southern Blotting demonstrated that the GAI gene was transferred into the H. brasiliensis genome successfully.