水稻内生真菌Fusarium moniliform ZSU-1次级代谢产物研究

Secondary Metabolites of the Endophtic Fungus Fusarium moniliforme ZSU-1 from Rice

摘要:

为了探索内生镰刀菌对稻田病害生物防治的物质基础，对一株来自健康水稻茎部的串珠镰刀菌Fusarium moniliform ZSU-1进行发酵培养，用色谱技术分别对其菌体提取物、发酵液提取物进行活性次级代谢产物分离，得到4个化合物，通过1D、2D NMR、质谱等波谱分析，分别鉴定为镰刀菌酸(1)、镰刀菌酸•脱氢镰刀菌酸缔合物(1: 1)(2)、白僵菌素(3)和甘露醇(4)。鉴于镰刀菌酸、镰刀菌酸•脱氢镰刀菌酸缔合物(1: 1)有较强的抗菌活性，推测这两个化合物对稻田病害生物防治可能有一定的意义。

Abstract:

The preponderant endophytic fungi of rice in Guangdong Province were Fusarium spp. In order to study the function of secondary metabolites from endophytic fungi on bio-control to rice paddy disease, a strain of endophtic fungus Fusarium moniliform ZSU-1 from sterily rice stem was ferment cultured, four secondary metabolites was isolated and purified by silica column and sephadex LH-20 chromatogram. On the basis of 1D、2D NMR and mass spectral data, there were fusaric acid (1), fusaric and 9,10-dehydrofusaric acids(1: 1) (2), beauvericin (3) and mannitol (4). The components 1 and 2 have strong antibacterial activities, they might be used for bio-control to rice paddy disease.