稻瘟病菌组蛋白脱乙酰化酶SIR2 HDACs的功能初探

Functional Analysis of Histone Deacetylase SIR2 HDACs Family in Magnaporthe grisea

摘要:

利用生物信息学方法对稻瘟病菌(Magnaporthe grisea)组蛋白脱乙酰化酶SIR2家族成员(SRT3001、SRT3002、SRT3003、SRT3004、SRT3005、SRT3006和SRT3007)的生物学功能进行研究。结果表明，SIR2 HDACs家族成员位于不同的染色体上，分布于细胞的不同部位；它们编码的蛋白均含有蛋白激酶C磷酸化、酪蛋白激酶II磷酸化、N-糖基化、N-豆蔻酰化4个相同的位点；它们均无跨膜区，且为亲水蛋白质，都无信号肽。多序列比对发现稻瘟病菌SIR2 HDACs家族结构域高度保守，均含有SIR2结构域；这些蛋白可能参与病原菌的转录调节、能量代谢和染色质沉默等过程。

Abstract:

The silent information regulator protein (Sir2) and its homologues collectively known as Sirtuins, NAD+-dependent deacetylase enzymes, play an important role in physiological regulation, such as the survival, aging and apoptosis of cell. The biological function of SIR2 HDACs family from Magnaporthe grisea was investigated by using bioinformatics. The results showed that the members of SIR2 family were localized in the different chromosomes and different parts of Magnaporthe grisea cell, and their encoding proteins all have four sites, such as the protein kinase C phosphorylation site, the Casein kinase II phosphorylation site, the N-glycosylation site, and the N-myristoylation site. The SIR2 HDACs members all haven’t transmembrane regions and they are hydrophilic proteins without signal peptide. The SIR2 HDACs members from M. grisea were aligned by ClustalW2 and their SIR2 domains are highly conserved. These SIR2 HDACs seemed to be involved in processes, such as transcription regulation, energy metabolism and chromatin silencing in M. grisea.