龙眼花芽与叶芽差异蛋白分析

Analysis of Differential Proteins during Development Stages of Flower Bud and Leaf Bud in Longan

摘要:

以龙眼(Dimocarpus longan Lour.)花芽和叶芽为材料，对龙眼花芽与叶芽分化发育过程中的差异蛋白质进行分析和鉴定，并探讨其生物学功能。本研究共鉴定出10个差异蛋白质：ATP synthase beta subunit，fructokinase，LHCII type I chlorophyll a/b binding protein，peroxidase， ascorbate peroxidase，14-3-3 protein，14-3-3 family protein，putative cytosolic cysteine synthase 7，retrotransposon protein，putative，Ty1-copia subclass，Protein Group similar to late embryogenesis abundant proteins。这些差异蛋白质可能与龙眼花芽与叶芽的物质和能量代谢、自由基清除和抗氧化作用、信号转导和基础代谢、氨基酸代谢等生理过程密切相关。

Abstract:

The proteomes during development stages of flower bud and leaf bud in longan (Dimocarpus longan Lour.) were isolated and identified by using 2-DE technology and MS analysis. Ten proteins were identified by MALDI-TOF-TOF/MS, such as ATP synthase beta subunit, fructokinase, LHCII type I chlorophyll a/b binding protein, peroxidase, ascorbate peroxidase, 14-3-3 protein, 14-3-3 family protein, putative cytosolic cysteine synthase 7, retrotransposon protein, putative, Ty1-copia subclass, protein group similar to late embryogenesis abundant proteins. These proteins might involve in substance and energy metabolism, scavenging and anti-oxidation of free radical, signal transduction and basic metabolism, amino acid metabolism and other physiological processes.