吊钟花的组织培养技术研究

Tissue Culture and Rapid Propagation of Enkianthus quinqueflorus Lour

摘要:

以吊钟花(Enkianthus quinqueflorus Lour.)茎尖及带腋芽的茎段为外植体进行组织培养技术研究。结果表明，改良B5培养基(B5大量元素和钙盐+MS有机物、铁盐、微量元素)最有利于吊钟花的培养, 外植体在改良B5+2,4-D 1 mg L-1的培养基中，愈伤组织诱导率可达100%。在含BA 1∼2 mg L-1+NAA0.1∼0.5 mg L-1培养基中，可诱导产生不定芽。继代培养以改良B5+BA 1 mg L-1+NAA 0.5 mg L-1培养基的增殖系数最高。生根培养基以1/2MS+IBA 2 mg L-1为最佳，生根率可达80%以上。试管苗移栽成活率为90%以上。

Abstract:

The rapid propagation of Enkianthus quinqueflouus Lour. had been established by using shoot-tip or stem with auxiliary buds as explants. The modified B5 medium as basal medium was optimum for callus induction. The callus induction rate was up to 100% cultured on B5 medium with 1 mg L-1 2,4-D. The adventitious buds were induced on B5 medium supplemented with 1∼2 mg L-1 BA +0.1∼0.5 mg L-1 NAA. Propagation coefficient was the highest when adventitious buds were subcultured on modified B5+1 mg L-1 BA+0.5 mg L-1 NAA. The rooting could reach 80% when adventitious buds cultured on 1/2MS+2 mg L-1 IBA, and the survival of plantlet could reach 90% after transferred into perlite bed.