农杆菌介导的MpASR蛋白在洋葱表皮细胞的定位研究

Studies on Subcellular Localization of MpASR in Onion Epidermal Cells Mediated by Agrobacterium

摘要:

通过比较不同侵染液浓度、侵染时间、取材部位、预处理等条件下的转化效果，优化了农杆菌介导的洋葱鳞茎内表皮细胞转化系统，并且成功检测到大蕉ASR蛋白(MpASR)与GFP的融合蛋白在洋葱表皮细胞中的分布。

关键词:MpASR蛋白;亚细胞定位;洋葱;内表皮细胞;绿色荧光蛋白;农杆菌

Abstract:

The effects of several factors on onion (Allium cepa L.) epidermal cells transformation were studied, including concentration of infection solution, infection time, parts of material and pre-treatment. The transformation system for onion epidermal cells mediated by Agrobacterium was established. The location of MpASR from Musa sapientum fused with GFP in onion epidermal cells was detected successfully.

Key words:MpASR; Subcellular localization; Onion; Epidermic cell; GFP; Agrobacterium