农杆菌介导的MpASR蛋白在洋葱表皮细胞的定位研究

Studies on Subcellular Localization of MpASR in Onion Epidermal Cells Mediated by Agrobacterium

摘要:

通过比较不同侵染液浓度、侵染时间、取材部位、预处理等条件下的转化效果，优化了农杆菌介导的洋葱鳞茎内表皮细胞转化系统，并且成功检测到大蕉ASR蛋白(MpASR)与GFP的融合蛋白在洋葱表皮细胞中的分布。

Abstract:

The effects of several factors on onion (Allium cepa L.) epidermal cells transformation were studied, including concentration of infection solution, infection time, parts of material and pre-treatment. The transformation system for onion epidermal cells mediated by Agrobacterium was established. The location of MpASR from Musa sapientum fused with GFP in onion epidermal cells was detected successfully.