假鞭叶铁线蕨配子体发育的研究

Gametophyte Development of Adiantum malesianum Chatak

摘要:

用1/2MS培养基培养假鞭叶铁线蕨(Adiantum malesianum Chatak)孢子，光学显微镜下观察孢子萌发及配子体发育过程。结果表明：成熟的孢子棕红色，不透明，四面体型，辐射对称，三裂缝。接种6 d左右孢子萌发，萌发类型为紫萁型(Osmunda-type)，原叶体发育为铁线蕨型(Adiantum-type)。接种25 d左右片状体形成。接种50 d左右发育为成熟原叶体，呈心脏形，裸露无毛状体。接种60 d左右性器官出现，雌雄同株。精子器长圆球形，由3细胞构成；成熟颈卵器颈部由4列细胞组成，3~5层细胞高。

Abstract:

The spores of Adiantum malesianum Chatak were cultured in 1/2MS medium. The spore germination and gametophyte development were observed by light microscope. The results showed that the mature spores were brown-red, opaque, tetrahedral type, actinomorphous, trilete. The spores germinated about six days after sowing. The spore germination belonged to the Osmunda-type, while the gametophyte development was of the Adiantum-type. The prothallial plates formed about 25 days after sowing. The mature cordate prothallus developed 50 days after sowing, gymnotremoid without trichomes. The sexual organs appeared 60 days after sowing, hermaphroditic. The antheridium was prolate spheroidal, consisting of three cells. Archegonial neck was usually composed of four rows of cells and three to five cells high at maturity.