切割方式和外植体大小对蝴蝶兰叶片诱导原球茎的影响

Effects of Cutting and Explant Size on PLB Induction from Phalaenopsis amabilis Leaves

摘要:

以蝴蝶兰(Phalaenopsis amabilis)试管播种苗的叶片为外植体, 以1/2MS + 10%椰乳+ NAA 0.1 mg L-1+TDZ 1.0 mg L-1+0.5%琼脂为诱导培养基, 研究了切割方式和外植体大小对类原球茎(protocorm-like body, PLB)诱导的影响。结果显示, 叶片经横切2刀和纵切2刀后, 叶片PLB诱导率和每叶片诱导出的PLB数量均极显著提高, PLB形成时间极显著缩短, 且横切处理的效果比纵切的更好; 叶片经横切成三分片段、六分片段和十二分片段后, 3种片段的PLB诱导率均极显著高于未切处理(对照), PLB形成时间均极显著短于对照, 且六分片段的PLB诱导率极显著高于其它两种片段。这些表明, 切割方式和外植体大小均对PLB的诱导有显著影响。

Abstract:

The leaves of Phalaenopsis amabilis were used as explants, cultured 1/2MS medium supplemented with 0.1 mg L-1 α-naphthyl acetic acid (NAA), 1.0 mg L-1 thidiazuron (TDZ), 10% coconut milk (CM)(v/v) to induce PLB (protocorm-like body), the effects of cutting and explant size on PLB induction were studied. The results showed that the leaves were cut transversely or longitudinally twice, both induction rate and average PLB number per leaf increased significantly, and the time of PLB formation decreased significantly. The effect of cutting transversely was better than that of cutting longitudinally. When the leaves were divided transversely into three, six and twelve segments, respectively, the PLB induction rates increased significantly and the time of PLB formation decreased significantly compared to the control. The PLB induction rate of six-divided segments was significantly higher than that of the three- and twelve-divided segments. These results showed that cutting and explant size could affect PLB induction from leaves of P. anabilis significantly.