不同地理种源杉木的分子多态性分析

Molecular Polymorphic Analysis for Different Geographic Provenances of Chinese Fir

摘要:

利用ISSR分子标记技术和筛选出的22条ISSR引物，对24个不同地理种源杉木的DNA进行扩增，共扩增出188条谱带，其中多态条带173条，占总数的92.0％。经计算，平均位点的有效等位基因是1.3408，Nei’s基因多样性指数是0.2154，Shannon多态性信息指数为0.3458，表明不同杉木种源间具有较高的遗传多样性。通过UPGMA法聚类分析，可把24个种源杉木分为五个类群：中带东区生态型、中带东南区生态型、中带中区生态型、南带生态型和北带生态型，表明杉木地理种源遗传距离聚类呈现出一定的地域性分布规律。

Abstract:

Genetic diversity of 24 geographic provenances of Chinese fir (Cunninghamia lanceolata) was analysed by ISSR (Inter-simple sequence repeat) markers. Twenty-two primers were screened from 100 ISSR primers. 188 DNA bands were amplified from 24 provenances of Chinese fir, 173 bands of which belonged to polymorphic bands, account for 92.0％. The effective number of alleles (Ne) was 1.3408，Nei’s index of genetic diversity was 0.2154 and Shannon’s index of polymorphic information was 0.3458. It indicated that the different geographic provenances of Chinese fir had higher genetic diversity. Twenty-four provenances of Chinese fir were divided into five groups by UPGMA cluster analysis: Eastern Area Ecotype in Middle Zone, Southeastern Area Ecotype in Middle Zone, Middle Area Ecotype in Middle Zone, Southern Zone Ecotype, Northern Zone Ecotype. This showed that the genetic distances of different geographic provenances of Chinese fir present regular geographical distribution.