人工合成启动子SAR在拟南芥中的表达分析

Study on Expression Pattern of the Synthetic Promoters SAR in Arabidopsis

摘要:

利用植物防御基因中的病原诱导响应元件和最小35S启动子(-62～+1)，人工合成了启动子SAR，并以GUS基因为报告基因，在转基因拟南芥中分析了合成启动子的表达特性。通过对转基因拟南芥GUS组织染色的分析表明：SAR启动子在子叶、毛刺、根茎交接处和根系中优势表达，在老叶中的表达量高于幼叶，说明SAR启动子具有组织和发育表达特异性。

Abstract:

The promoter SAR by combining mini-35S promoter (-62 to +1) and several pathogen responsive cis-acting elements derived from defense genes in plant was synthesized, and the expression pattern of these synthetic promoters was studied by using report gene GUS (β-glucuronidase) transformed into Arabidopsis. The results showed that synthetic promoter SAR could make GUS predominant expression in cotyledon, trichome, rhizome conjoit region, root system and old leaves, and the expression in old leaves was higher than that in young leaves. It suggested that SAR promoter had tissue and development specific expression.