桂热杧系列品种（系）的亲缘关系分析

Analysis on the Genetic Relationship of Gui Re Mango (Mangifera indica L) Cultivars or Lines

摘要:

从100条ISSR引物中选取12条多态性好的引物，对广西亚热带作物所选育的16个杧果(Mangifera indica L.)品种(系)及其可能亲本共22个样品的亲缘关系进行了分析。结果表明：这12条引物共扩增出127条DNA谱带，其中多态性条带为85条，条带多态百分率为66.93%。聚类分析显示，所有供试品种(系)之间的亲缘关系都比较近, 相似系数在0.81以上；如果以相似系数0.82为标准，可将供试的22个品种(系)分为3大类。利用ISSR技术能较好地区分桂热杧系列品种(系)的亲缘关系。

Abstract:

Twelve ISSR primers screened from 100 primers, were selected to analysis the genetic relationship of 22 materials belonging to 16 mango (Mangifera indica L.) cultivars or lines selected and breeded by the Guangxi Institute of Subtropical Crops. The results showed that 85 scorable polymorphic bands of a total 127 bands were produced and percentage of polymorphic bands was 66.93%. Based on UPGMA analysis, all samples showed higher similarities and their coefficients were more than 0.81. If the coefficient 0.82 was regarded as divided line of their genetic relationship, 22 materials could be clustered into three groups by ISSR. The genetic relationship of Gui Re Mango cultivars or lines could be better assessed by ISSR-PCR.