醮核荔枝胚胎发育相关蛋白质的分离及初步鉴定

Isolation and Identification of Proteins Related with Embryo Development in Seedless Litchi

摘要:

通过二维聚丙烯酰胺凝胶电泳以及计算机辅助的图像分析技术, 对荔枝开花后40 d的正常与败育胚蛋白质图谱进行初步分析。结果表明, 100个蛋白质点在表达丰度上有明显差异; 选择仅在正常发育胚胎胶上表达的蛋白点15个和仅在败育胚胎胶上表达的蛋白点50个, 进行基质辅助激光解吸附电离飞行时间质谱(MALDI-TOF-MASS)分析, 鉴定出9个与胚发育相关的蛋白, 这些蛋白可能参与了胚败育的调节和控制。

Abstract:

The global protein patterns in normal and aborted embryo of litchi at 40 days after flowering (DAA) were compared by using two-dimensional polyacrylamide gel electrophoresis (2-DE) and computer image analysis. The results showed that the quantities of 100 protein spots in normal embryo were significantly different from aborted embryo. There were 15 and 50 spots appeared on the gel of normal embryo and aborted embryo, respectively. Then they were selected for MALDI-TOF-MASS analysis. Nine proteins related with embryo development were identified, which maight involve in regulating and controlling the embryo abortion.