人乳铁蛋白在转基因马铃薯块茎中的表达

Expression of Human Lactoferrin in Transgenic Potato Tubers

摘要:

人乳铁蛋白(human lactoferrin, hLF)是人体非特异性免疫系统的重要成员之一，具有抗细菌、真菌和抗病毒活性及其他多种功能。报道将hLF基因的cDNA与马铃薯(Solanum tuberosum L.)块茎专一性表达patatin基因启动子融合后通过农杆菌介导导入马铃薯，PCR检测证实获得了多个转基因株系，RT-PCR阳性结果说明hLF mRNA在马铃薯植株中得到了表达。同时，经过ELISA及Western blot检测证实，转基因马铃薯表达了hLF并具有人乳铁蛋白的活性。

Abstract:

Human lactoferrin (hLF) is a member of non-specific immune system, it plays many roles, such as resistances against bacteria, fungi and viruses, etc. In this paper, hLF cDNA directed by the tuber specific promoter of class I patatin gene of potato (Solanum tuberosum L.) were transferred into potato mediated by Agrobacterium tumefiens transformation. hLF transgenic potato plants were confirmed by PCR detection, further RT-PCR showed that hLF RNAs were expressed in transgenic plants. hLF activities were detected by ELISA and Western blot analysis in hLF transgenic potato tubers.