沙田柚配子体自交不亲和花柱消减文库的构建及分析

Construction and Analysis of Suppression Subtractive Hybridization Library Related to Gametophytic Self-incompatibility in Style of Citrus grandis var. shatinyu Hort

摘要:

分别以沙田柚自交花柱cDNA为tester, 异交花柱cDNA为driver, 利用抑制性消减杂交技术构建了消减文库，文库的重组率高于95%，插入片段集中在100~500 bp之间，对文库部分克隆进行测序并与GenBank中的同源序列进行比较，发现了一些类似于S1、S9-RNase、激酶类等与自交不亲和相关的基因。

Abstract:

A suppression subtractive hybridization library of Citrus grandis var. shatinyu Hort was constructed by using the cDNA of self-pollinated styles as the tester and cross-pollinated styles as the driver. In the constructed library, the rate of recombination was more than 95% and the size of inserts ranged from 100 bp to 500 bp. After the partial clones of the library were sequenced and compared with homologous sequences in GenBank Database, some genes related with self-incompatibility were obtained, such as S1, S9-RNase and kinase.