费约果外植体灭菌及愈伤组织的诱导

Sterilization of the Explant and Callus Induction of Feijoa sallowiana Berg. in vitro Culture

摘要:

以费约果(Feijoa sallowiana Berg.)嫩叶和带芽茎段作为外植体，研究费约果愈伤组织诱导的最佳条件和方法。结果表明，(1)最佳基本培养基是MS；(2)二步灭菌法优于一步灭菌法；(3)嫩叶和带芽茎段愈伤组织诱导的最佳活性炭浓度分别为2.0 g L-1和3.0 g L-1；(4)最佳青霉素浓度分别为200 mg L-1和50 mg L-1；(5)光培养方式优于暗培养方式；(6)费约果嫩叶诱导愈伤组织的最佳培养基是MS+2.0 mg L-1 2,4-D+1.0 mg L-1 KT(或0.1 mg L-1 NAA)；(7)带芽茎段的最佳培养基是MS+1.0 mg L-1 6-BA+0.5 mg L-1 NAA+1.0 mg L-1 GA或MS+2.0 mg L-1 6-BA+0.1 mg L-1 NAA+1.0 mg L-1 GA。

Abstract:

The optimum conditions for the tissue culture of Feijoa (Feijoa sallowiana Berg.) were investigated. The leaves and shoot with bud were used as explants. The results showed that the optimal basal medium was MS medium. Two-step sterilization was better than one-step sterilization. The optimal concentration of active carbon for callus induction from leaves and shoot with bud were 2.0 g L-1 and 3.0 g L-1, respectively, and penicillin were 200 mg L-1 and 50 mg L-1, respectively. The culture under light was better than under dark. The optimal medium for callus induction from tender leaves was MS+2.0 mg L-1 2,4-D+1.0 mg L-1 KT (or 0.1 mg L-1 NAA), and from shoot with bud was MS+1.0 mg L-1 6-BA+0.5mg L-1 NAA+1.0 mg L-1 GA, or MS+2.0 mg L-1 6-BA+0.1 mg L-1 NAA+1.0 mg L-1 GA.