Chemical constituents of the liposoluble fraction from Antiaris toxicaria latex were analyzed by gas chromatography-mass spectrometry for the first time. Twenty-seven compounds were identified, amounting to 91.7% of the total detected constituents. Bioassay result showed the liposoluble fraction of A. toxicaria possessed antioxidant activity by DPPH free radical scavenging method, with SC50 value of 500 μg ml-1.